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The Effects of Insufflation Conditions on Rat Mesothelium
Joint Authors
Hayward, Jessica
Woods, Anthony E.
Davey, Andrew K.
Marshall, Jean K.
Source
International Journal of Inflammation
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Aim.
The aim of this investigation was to examine the alterations in the peritoneum after cold dry CO2, heated dry CO2, and humidified heated CO2 at pressures equivalent to intraperitoneal pressures used in human laparoscopy.
Methods.
Eighteen rats were divided into 4 treatment groups—group 1: untreated control; group 2: insufflation with cold dry CO2; group 3: insufflation with heated, dry CO2; group 4: insufflation with heated and humidified CO2.
The abdomen was insufflated to 5 mm/Hg (flow rate 50 mL/min) for 2 h.
Twelve hours later, tissue samples were collected for analysis by light microscopy (LM) and scanning electron microscopy (SEM).
Results.
Group 1: no abnormalities were detected.
Group 2: specimens revealed an inflammatory response with loss of mesothelium and mesothelial cell nuclei showing lytic change.
Cells were rounded with some areas of cell flattening and separation.
Group 3: some animals showed little or no alteration, while others had a mild inflammatory response.
Mesothelial cells were rounded and showed crenation on the exposed surface.
Group 4: specimens showed little change from the control group.
Conclusions.
The LM results indicate that insufflations with heated, humidified CO2 are the least likely to induce mesothelial damage.
American Psychological Association (APA)
Davey, Andrew K.& Hayward, Jessica& Marshall, Jean K.& Woods, Anthony E.. 2013. The Effects of Insufflation Conditions on Rat Mesothelium. International Journal of Inflammation،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-500390
Modern Language Association (MLA)
Davey, Andrew K.…[et al.]. The Effects of Insufflation Conditions on Rat Mesothelium. International Journal of Inflammation No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-500390
American Medical Association (AMA)
Davey, Andrew K.& Hayward, Jessica& Marshall, Jean K.& Woods, Anthony E.. The Effects of Insufflation Conditions on Rat Mesothelium. International Journal of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-500390
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500390