Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis
Joint Authors
Piriya, P. Sobana
Padma, V. S.
Vasan, P. Thirumalai
Archana, K.
Vidhyadevi, U.
Vennison, S. John
Source
Biotechnology Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Information Technology and Computer Science
Abstract EN
The ethanol fermenting genes such as pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adh II) were cloned from Zymomonas mobilis and transformed into three different cellulolytic bacteria, namely Enterobacter cloacae JV, Proteus mirabilis JV and Erwinia chrysanthemi and their cellulosic ethanol production capability was studied.
Recombinant E.
cloacae JV was found to produce 4.5% and 3.5% (v/v) ethanol, respectively, when CMC and 4% NaOH pretreated bagasse were used as substrates, whereas recombinant P.
mirabilis and E.
chrysanthemi with the same substrates could only produce 4%, 3.5%, 1%, and 1.5 % of ethanol, respectively.
The recombinant E.
cloacae strain produced twofold higher percentage of ethanol than the wild type.
The recombinant E.
cloacae strain could be improved further by increasing its ethanol tolerance capability through media optimization and also by combining multigene cellulase expression for enhancing ethanol production from various types of lignocellulosic biomass so that it can be used for industrial level ethanol production.
American Psychological Association (APA)
Piriya, P. Sobana& Vasan, P. Thirumalai& Padma, V. S.& Vidhyadevi, U.& Archana, K.& Vennison, S. John. 2012. Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis. Biotechnology Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-500511
Modern Language Association (MLA)
Piriya, P. Sobana…[et al.]. Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis. Biotechnology Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-500511
American Medical Association (AMA)
Piriya, P. Sobana& Vasan, P. Thirumalai& Padma, V. S.& Vidhyadevi, U.& Archana, K.& Vennison, S. John. Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis. Biotechnology Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-500511
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500511