High-Frequency Regeneration of the Drought-Tolerant Tree Melia volkensii Gurke Using Low-Cost Agrochemical Thidiazuron
Joint Authors
Akunda, Elijah
Kinyamario, Jenesio I.
Adero, Mark Ochieng
Mulanda, Eliud Sagwa
Amugune, Nelson Onzere
Source
Biotechnology Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-27
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Information Technology and Computer Science
Abstract EN
Melia volkensii Gurke is a drought-tolerant tree native to East Africa’s arid and semiarid lands (ASALs), with vast but underutilized potential for agroforestry and sustainable livelihoods in the ASALs.
Its cultivation is limited by difficulties in propagation via conventional means.
Full exploitation of the ability of thidiazuron (TDZ) to elicit regeneration in plant tissue cultures, as sole plant growth regulator (PGR), is hampered by high costs.
This study tested the effectiveness of a low-cost agrochemical TDZ for in vitro propagation of M.
volkensii.
Zygotic embryos from mature seeds were cultured on Gamborg’s B5 medium containing 0 to 4 mg/L of agrochemical TDZ from Kingtai Chemicals Co.,Ltd., China.
Callus induction frequency was 96.67 to 100%.
Significantly large callus fresh mass was produced at 0.05 mg/L TDZ concentration (ANOVA, P<0.001).
The effect of TDZ on embryogenicity was significant over certain ranges of concentrations (Anova, P<0.001).
Multiple somatic embryos developed within 14 days of subculture to hormone-free B5 medium.
Somatic embryos developed into microshoots which elongated when transferred to 1/2 MS medium supplemented with 0.1 mg/L 6-benzylaminopurine plus 10% coconut water.
The Kingtai-TDZ showed a high potency and suitability for use in M.
volkensii tissue culture.
American Psychological Association (APA)
Mulanda, Eliud Sagwa& Adero, Mark Ochieng& Amugune, Nelson Onzere& Akunda, Elijah& Kinyamario, Jenesio I.. 2012. High-Frequency Regeneration of the Drought-Tolerant Tree Melia volkensii Gurke Using Low-Cost Agrochemical Thidiazuron. Biotechnology Research International،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-500574
Modern Language Association (MLA)
Mulanda, Eliud Sagwa…[et al.]. High-Frequency Regeneration of the Drought-Tolerant Tree Melia volkensii Gurke Using Low-Cost Agrochemical Thidiazuron. Biotechnology Research International No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-500574
American Medical Association (AMA)
Mulanda, Eliud Sagwa& Adero, Mark Ochieng& Amugune, Nelson Onzere& Akunda, Elijah& Kinyamario, Jenesio I.. High-Frequency Regeneration of the Drought-Tolerant Tree Melia volkensii Gurke Using Low-Cost Agrochemical Thidiazuron. Biotechnology Research International. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-500574
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500574