Frequency of Drug Resistance Gene Amplification in Clinical Leishmania Strains

Joint Authors

Deniau, M.
Aoun, K.
Dereure, J.
Faraut, F.
Mary, Charles
Piarroux, R.
Ranque, S.

Source

International Journal of Microbiology

Issue

Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2010-07-12

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Natural & Life Sciences (Multidisciplinary)
Medicine

Abstract EN

Experimental studies about Leishmania resistance to metal and antifolates have pointed out that gene amplification is one of the main mechanisms of drug detoxification.

Amplified genes code for adenosine triphosphate-dependent transporters (multidrug resistance and P-glycoproteins P), enzymes involved in trypanothione pathway, particularly gamma glutamyl cysteine synthase, and others involved in folates metabolism, such as dihydrofolate reductase and pterine reductase.

The aim of this study was to detect and quantify the amplification of these genes in clinical strains of visceral leishmaniasis agents: Leishmania infantum, L.

donovani, and L.

archibaldi.

Relative quantification experiments by means of real-time polymerase chain reaction showed that multidrug resistance gene amplification is the more frequent event.

For P-glycoproteins P and dihydrofolate reductase genes, level of amplification was comparable to the level observed after in vitro selection of resistant clones.

Gene amplification is therefore a common phenomenon in wild strains concurring to Leishmania genomic plasticity.

This finding, which corroborates results of experimental studies, supports a better understanding of metal resistance selection and spreading in endemic areas.

American Psychological Association (APA)

Mary, Charles& Faraut, F.& Deniau, M.& Dereure, J.& Aoun, K.& Ranque, S.…[et al.]. 2010. Frequency of Drug Resistance Gene Amplification in Clinical Leishmania Strains. International Journal of Microbiology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-500628

Modern Language Association (MLA)

Mary, Charles…[et al.]. Frequency of Drug Resistance Gene Amplification in Clinical Leishmania Strains. International Journal of Microbiology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-500628

American Medical Association (AMA)

Mary, Charles& Faraut, F.& Deniau, M.& Dereure, J.& Aoun, K.& Ranque, S.…[et al.]. Frequency of Drug Resistance Gene Amplification in Clinical Leishmania Strains. International Journal of Microbiology. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-500628

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-500628