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Effects of Composition of Iron-Cross-Linked Alginate Hydrogels for Cultivation of Human Dermal Fibroblasts
Joint Authors
Satoh, Nao
Machida-Sano, Ikuko
Kimura, Yoshitaka
Namiki, Hideo
Ueda, Hiroyuki
Ogawa, Sakito
Source
International Journal of Biomaterials
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We investigated the suitability of ferric-ion-cross-linked alginates (Fe-alginate) with various proportions of L-guluronic acid (G) and D-mannuronic acid (M) residues as a culture substrate for human dermal fibroblasts.
High-G and high-M Fe-alginate gels showed comparable efficacy in promoting initial cell adhesion and similar protein adsorption capacities, but superior cell proliferation was observed on high-G than on high-M Fe-alginate as culture time progressed.
During immersion in culture medium, high-G Fe-alginate showed little change in gel properties in terms of swelling and polymer content, but the properties of high-M Fe-alginate gel were altered due to loss of ion cross-linking.
However, the degree of cell proliferation on high-M Fe-alginate gel was improved after it had been stabilized by immersion in culture medium until no further changes occurred.
These results suggest that the mode of cross-linkage between ferric ions and alginate differs depending on alginate composition and that the major factor giving rise to differences in cell growth on the two types of Fe-alginate films is gel stability during culture, rather than swelling of the original gel, polymer content, or protein adsorption ability.
Our findings may be useful for extending the application of Fe-alginate to diverse biomedical fields.
American Psychological Association (APA)
Machida-Sano, Ikuko& Ogawa, Sakito& Ueda, Hiroyuki& Kimura, Yoshitaka& Satoh, Nao& Namiki, Hideo. 2012. Effects of Composition of Iron-Cross-Linked Alginate Hydrogels for Cultivation of Human Dermal Fibroblasts. International Journal of Biomaterials،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-500760
Modern Language Association (MLA)
Machida-Sano, Ikuko…[et al.]. Effects of Composition of Iron-Cross-Linked Alginate Hydrogels for Cultivation of Human Dermal Fibroblasts. International Journal of Biomaterials No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-500760
American Medical Association (AMA)
Machida-Sano, Ikuko& Ogawa, Sakito& Ueda, Hiroyuki& Kimura, Yoshitaka& Satoh, Nao& Namiki, Hideo. Effects of Composition of Iron-Cross-Linked Alginate Hydrogels for Cultivation of Human Dermal Fibroblasts. International Journal of Biomaterials. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-500760
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500760