Fibronectin Binding Is Required for Acquisition of MesenchymalEndothelial Differentiation Potential in Human Circulating Monocytes

Abstract EN

We previously reported monocyte-derived multipotential cells (MOMCs), which include progenitors capable of differentiating into a variety of mesenchymal cells and endothelial cells.

In vitro generation of MOMCs from circulating CD14+ monocytes requires their binding to extracellular matrix (ECM) protein and exposure to soluble factor(s) derived from circulating CD14- cells.

Here, we investigated the molecular factors involved in MOMC generation by examining the binding of monocytes to ECM proteins.

We found that MOMCs were obtained on the fibronectin, but not on type I collagen, laminin, or poly-L-lysine.

MOMC generation was followed by changes in the expression profiles of transcription factors and was completely inhibited by either anti-α5 integrin antibody or a synthetic peptide that competed with the RGD domain for the β1-integrin binding site.

These results indicate that acquisition of the multidifferentiation potential by circulating monocytes depends on their binding to the RGD domain of fibronectin via cell-surface α5β1 integrin.