D-Glucosamine Conjugation Accelerates the Labeling Efficiency of Quantum Dots in Osteoblastic Cells
Joint Authors
Yamada, Shizuka
Hayashi, Yoshihiko
Xie, Ming-Fang
Ohba, Hideki
Igawa, Kazunari
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-24
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Quantum dots (QDs) are useful imaging tools in the medical and biological fields due to their optical properties, such as a high fluorescence intensity, remarkable resistance to photobleaching, broad absorption spectra, and narrow emission spectra.
This is the first study to investigate the uptake of carboxylated QDs conjugated with D-glucosamine (core size: approximately 3 nm, final modified size: 20–30 nm) into cultured osteoblastic cells.
The QDs attached to the cell surface and were transported into the cytoplasm within approximately three hours of culture, whose process was clearly demonstrated using specific fluorescent staining of the cell membrane.
Although the intranuclear distribution was not observed, a dramatic decrease in the transfer of quantum dots into the cytoplasm was recognized after approximately seven days of culture.
Other interesting phenomena include the escape of the quantum dots from lysosomes in the cytoplasm, as confirmed by the merging of both QD fluorescence and specific fluorescent staining of lysosomes in the cytoplasm.
These findings suggest that D-glucosamine conjugation enhances proton absorption in acid organelles and promotes the lysosomal escape of QDs.
American Psychological Association (APA)
Igawa, Kazunari& Xie, Ming-Fang& Ohba, Hideki& Yamada, Shizuka& Hayashi, Yoshihiko. 2014. D-Glucosamine Conjugation Accelerates the Labeling Efficiency of Quantum Dots in Osteoblastic Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-500860
Modern Language Association (MLA)
Igawa, Kazunari…[et al.]. D-Glucosamine Conjugation Accelerates the Labeling Efficiency of Quantum Dots in Osteoblastic Cells. BioMed Research International No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-500860
American Medical Association (AMA)
Igawa, Kazunari& Xie, Ming-Fang& Ohba, Hideki& Yamada, Shizuka& Hayashi, Yoshihiko. D-Glucosamine Conjugation Accelerates the Labeling Efficiency of Quantum Dots in Osteoblastic Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-500860
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500860