Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3 : Downregulation of Inflammatory and Regulatory Genes
Joint Authors
del Pozo, Victoria
Gámez, Cristina
Zafra, María Paz
Sastre, Beatriz
López, Esther
Lahoz, Carlos
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Asthma is a disorder characterized by a predominance of Th2 cells and eosinophilic inflammation.
Suppressors of cytokine signaling (SOCS) proteins act as negative regulators of cytokine signaling.
In particular, SOCS1 and SOCS3 play an important role in immune response by controlling the balance between Th1 and Th2 cells.
In a previous study, we demonstrated that treatment of chronic asthmatic mice with gene therapy using plasmid encoding galectin-3 (Gal-3) led to an improvement in Th2 allergic inflammation.
Methods.
Using a microarray approach, this study endeavored to evaluate the changes produced by therapeutic Gal-3 delivered by gene therapy in a well-characterized mouse model of chronic airway inflammation.
Results were confirmed by real-time RT-PCR, Western blot and immunohistochemical analysis.
Results.
We identify a set of genes involved in different pathways whose expression is coordinately decreased/increased in mice treated with Gal-3 gene therapy.
We report a correlation between Gal-3 treatment and inhibition of SOCS1 and SOCS3 expression in lungs.
Conclusion.
These results suggest that negative regulation of SOCS1 and 3 following Gal-3 treatment could be a valuable therapeutic approach in allergic disease.
American Psychological Association (APA)
López, Esther& Zafra, María Paz& Sastre, Beatriz& Gámez, Cristina& Lahoz, Carlos& del Pozo, Victoria. 2011. Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3 : Downregulation of Inflammatory and Regulatory Genes. Mediators of Inflammation،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-500937
Modern Language Association (MLA)
López, Esther…[et al.]. Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3 : Downregulation of Inflammatory and Regulatory Genes. Mediators of Inflammation No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-500937
American Medical Association (AMA)
López, Esther& Zafra, María Paz& Sastre, Beatriz& Gámez, Cristina& Lahoz, Carlos& del Pozo, Victoria. Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3 : Downregulation of Inflammatory and Regulatory Genes. Mediators of Inflammation. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-500937
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500937