A Critical Appraisal of Quantitative Studies of Protein Degradation in the Framework of Cellular Proteostasis
Joint Authors
Alvarez-Castelao, Beatriz
Castaño, José G.
Ruiz-Rivas, Carmen
Source
Biochemistry Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Protein homeostasis, proteostasis, is essential to understand cell function.
Protein degradation is a crucial component of the proteostatic mechanisms of the cell.
Experiments on protein degradation are nowadays present in many investigations in the field of molecular and cell biology.
In the present paper, we focus on the different experimental approaches to study protein degradation and present a critical appraisal of the results derived from steady-state and kinetic experiments using detection of unlabelled and labelled protein methodologies with a proteostatic perspective.
This perspective allows pinpointing the limitations in interpretation of results and the need of further experiments and/or controls to establish “definitive evidence” for the role of protein degradation in the proteostasis of a given protein or the entire proteome.
We also provide a spreadsheet for simple calculations of mRNA and protein decays for mimicking different experimental conditions and a checklist for the analysis of experiments dealing with protein degradation studies that may be useful for researchers interested in the area of protein turnover.
American Psychological Association (APA)
Alvarez-Castelao, Beatriz& Ruiz-Rivas, Carmen& Castaño, José G.. 2012. A Critical Appraisal of Quantitative Studies of Protein Degradation in the Framework of Cellular Proteostasis. Biochemistry Research International،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-500967
Modern Language Association (MLA)
Alvarez-Castelao, Beatriz…[et al.]. A Critical Appraisal of Quantitative Studies of Protein Degradation in the Framework of Cellular Proteostasis. Biochemistry Research International No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-500967
American Medical Association (AMA)
Alvarez-Castelao, Beatriz& Ruiz-Rivas, Carmen& Castaño, José G.. A Critical Appraisal of Quantitative Studies of Protein Degradation in the Framework of Cellular Proteostasis. Biochemistry Research International. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-500967
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500967