Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR
Joint Authors
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-21
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Thermus thermophilus MutS, a thermostable mismatch-recognizing protein, is utilized in PCR to suppress nonspecific amplification by preventing synthesis from mismatched primers.
T.
thermophilus RecA also decreases nonspecific amplification by promoting proper hybridization between the primer and template.
We observed that MutS and RecA function under the same reaction conditions and that MutS and RecA do not preclude each other.
Furthermore, there were some DNA sequences for which only one of the 2 proteins effectively suppressed nonspecific amplification.
The simultaneous use of MutS and RecA is a more attractive error-suppressing technique than the use of either of the 2 proteins alone.
American Psychological Association (APA)
Fukui, Kenji& Kuramitsu, Seiki. 2013. Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR. Journal of Nucleic Acids،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-500981
Modern Language Association (MLA)
Fukui, Kenji& Kuramitsu, Seiki. Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR. Journal of Nucleic Acids No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-500981
American Medical Association (AMA)
Fukui, Kenji& Kuramitsu, Seiki. Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR. Journal of Nucleic Acids. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-500981
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500981