A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application
Joint Authors
Singh, S. P.
Arya, Sunil K.
Saha, Shibu
Gupta, Vinay
Source
International Journal of Electrochemistry
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-20
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A novel hybrid matrix of zinc oxide-methylene blue (ZnO-MB) has been successfully developed for biosensing application.
The introduction of methylene blue into the ZnO thin film leads to reduction in the charge transfer resistance and suggests an increase in the electron transfer capacity of the composite.
Glucose oxidase (GOx) was chosen as the model enzyme and effectively immobilized on the surface of hybrid ZnO-MB nanocomposite matrix.
Electrochemical measurements were employed to study biosensing response of the GOx/ZnO-MB/ITO bioelectrode as a function of glucose concentration.
The low oxidation potential (−0.23 V) of the hybrid bioelectrode, in a mediatorless electrolyte, makes it resistant against interference from other bio-molecules.
The low value of Michaelis-Menten constant (2.65 mM) indicates that immobilized GOx retains its enzymatic activity significantly on the surface of nanocomposite hybrid matrix that results in an enhanced affinity towards its substrate (glucose).
The ZnO-MB nanocomposite hybrid matrix, exhibiting enhanced sensing response (0.2 μAmM−1cm−2) with long shelf-life (>10 weeks), has potential for the realization of an integrated biosensing device.
American Psychological Association (APA)
Saha, Shibu& Arya, Sunil K.& Singh, S. P.& Gupta, Vinay. 2011. A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application. International Journal of Electrochemistry،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-500982
Modern Language Association (MLA)
Saha, Shibu…[et al.]. A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application. International Journal of Electrochemistry No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-500982
American Medical Association (AMA)
Saha, Shibu& Arya, Sunil K.& Singh, S. P.& Gupta, Vinay. A Novel ZnO-Methylene Blue Nanocomposite Matrix for Biosensing Application. International Journal of Electrochemistry. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-500982
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500982