Coincident In Vitro Analysis of DNA-PK-Dependent and -Independent Nonhomologous End Joining
Joint Authors
Pastwa, Elzbieta
Winters, Thomas A.
Neumann, Ronald D.
Purkayastha, Shubhadeep
Hendrickson, Cynthia L.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
In mammalian cells, DNA double-strand breaks (DSBs) are primarily repaired by nonhomologous end joining (NHEJ).
The current model suggests that the Ku 70/80 heterodimer binds to DSB ends and recruits DNA-PKcs to form the active DNA-dependent protein kinase, DNA-PK.
Subsequently, XRCC4, DNA ligase IV, XLF and most likely, other unidentified components participate in the final DSB ligation step.
Therefore, DNA-PK plays a key role in NHEJ due to its structural and regulatory functions that mediate DSB end joining.
However, recent studies show that additional DNA-PK-independent NHEJ pathways also exist.
Unfortunately, the presence of DNA-PKcs appears to inhibit DNA-PK-independent NHEJ, and in vitro analysis of DNA-PK-independent NHEJ in the presence of the DNA-PKcs protein remains problematic.
We have developed an in vitro assay that is preferentially active for DNA-PK-independent DSB repair based solely on its reaction conditions, facilitating coincident differential biochemical analysis of the two pathways.
The results indicate the biochemically distinct nature of the end-joining mechanisms represented by the DNA-PK-dependent and -independent NHEJ assays as well as functional differences between the two pathways.
American Psychological Association (APA)
Hendrickson, Cynthia L.& Purkayastha, Shubhadeep& Pastwa, Elzbieta& Neumann, Ronald D.& Winters, Thomas A.. 2010. Coincident In Vitro Analysis of DNA-PK-Dependent and -Independent Nonhomologous End Joining. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-501005
Modern Language Association (MLA)
Hendrickson, Cynthia L.…[et al.]. Coincident In Vitro Analysis of DNA-PK-Dependent and -Independent Nonhomologous End Joining. Journal of Nucleic Acids No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-501005
American Medical Association (AMA)
Hendrickson, Cynthia L.& Purkayastha, Shubhadeep& Pastwa, Elzbieta& Neumann, Ronald D.& Winters, Thomas A.. Coincident In Vitro Analysis of DNA-PK-Dependent and -Independent Nonhomologous End Joining. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-501005
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501005