Nitro-Oxidative Stress after Neuronal Ischemia Induces Protein Nitrotyrosination and Cell Death
Joint Authors
Planas, Anna
Ois, Angel
Palomer, Ernest
Guix, Francesc X.
ILL-Raga, Gerard
Roquer, Jaume
Galán, Ana M.
Tajes, Marta
Reyes-Navarro, Mario
Guivernau, Biuse
Caballo, Carolina
Alameda, Francesc
Ramos-Fernández, Eva
Bosch-Morató, Mònica
Opazo, Carlos
Muñoz, Francisco J.
Escolar, Ginés
Valverde, Miguel A.
Pérez-Asensio, Fernando
Jiménez-Conde, Jordi
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Ischemic stroke is an acute vascular event that obstructs blood supply to the brain, producing irreversible damage that affects neurons but also glial and brain vessel cells.
Immediately after the stroke, the ischemic tissue produces nitric oxide (NO) to recover blood perfusion but also produces superoxide anion.
These compounds interact, producing peroxynitrite, which irreversibly nitrates protein tyrosines.
The present study measured NO production in a human neuroblastoma (SH-SY5Y), a murine glial (BV2), a human endothelial cell line (HUVEC), and in primary cultures of human cerebral myocytes (HC-VSMCs) after experimental ischemia in vitro.
Neuronal, endothelial, and inducible NO synthase (NOS) expression was also studied up to 24 h after ischemia, showing a different time course depending on the NOS type and the cells studied.
Finally, we carried out cell viability experiments on SH-SY5Y cells with H2O2, a prooxidant agent, and with a NO donor to mimic ischemic conditions.
We found that both compounds were highly toxic when they interacted, producing peroxynitrite.
We obtained similar results when all cells were challenged with peroxynitrite.
Our data suggest that peroxynitrite induces cell death and is a very harmful agent in brain ischemia.
American Psychological Association (APA)
Tajes, Marta& ILL-Raga, Gerard& Palomer, Ernest& Ramos-Fernández, Eva& Guix, Francesc X.& Bosch-Morató, Mònica…[et al.]. 2013. Nitro-Oxidative Stress after Neuronal Ischemia Induces Protein Nitrotyrosination and Cell Death. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-501190
Modern Language Association (MLA)
Tajes, Marta…[et al.]. Nitro-Oxidative Stress after Neuronal Ischemia Induces Protein Nitrotyrosination and Cell Death. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-501190
American Medical Association (AMA)
Tajes, Marta& ILL-Raga, Gerard& Palomer, Ernest& Ramos-Fernández, Eva& Guix, Francesc X.& Bosch-Morató, Mònica…[et al.]. Nitro-Oxidative Stress after Neuronal Ischemia Induces Protein Nitrotyrosination and Cell Death. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-501190
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501190