Nymphaeol-A Isolated from Okinawan Propolis Suppresses Angiogenesis and Induces Caspase-Dependent Apoptosis via Inactivation of Survival Signals
Joint Authors
Tsuchiya, Ikumi
Hosoya, Takahiro
Kunimasa, Kazuhiro
Kumazawa, Shigenori
Ushida, Motoko
Ohta, Toshiro
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Propolis, a resinous substance that honeybees collect to protect their beehive from enemies, is reported to have various biological activities.
In our screening program to search for antiangiogenic compounds from propolis, the ethanol extracts of Okinawan propolis (EEOP) showed significant antiangiogenic activities in a tube formation assay with human umbilical vein endothelial cells (HUVECs) in vitro at 3.13 μg/mL and chorioallantoic membrane (CAM) assay in vivo at 25 μg/egg.
To elucidate the active compounds of EEOP and their mode of action, we isolated some prenylated flavonoids from EEOP and found that nymphaeol-A had the strongest antiangiogenic activity among them.
Nymphaeol-A significantly reduced in vivo neovessel formation in the CAM assay at 25 μg/egg.
At the molecular level, nymphaeol-A markedly inactivated mitogen-activated protein kinase/ERK kinase 1/2 (MEK1/2) and extracellular signal-regulated kinase 1/2 (ERK1/2), whose molecular activations signal new vessel formation in HUVECs.
In addition, nymphaeol-A dose- and time-dependently induced caspase-dependent apoptosis in tube-forming HUVECs.
Taken together, nymphaeol-A was shown to inhibit angiogenesis at least in part via inactivation of MEK1/2–ERK1/2 signaling and induction of caspase-dependent apoptosis.
Okinawan propolis and its major component, nymphaeol-A, may be useful agents for preventing tumor-induced angiogenesis.
American Psychological Association (APA)
Tsuchiya, Ikumi& Hosoya, Takahiro& Ushida, Motoko& Kunimasa, Kazuhiro& Ohta, Toshiro& Kumazawa, Shigenori. 2013. Nymphaeol-A Isolated from Okinawan Propolis Suppresses Angiogenesis and Induces Caspase-Dependent Apoptosis via Inactivation of Survival Signals. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-501204
Modern Language Association (MLA)
Tsuchiya, Ikumi…[et al.]. Nymphaeol-A Isolated from Okinawan Propolis Suppresses Angiogenesis and Induces Caspase-Dependent Apoptosis via Inactivation of Survival Signals. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-501204
American Medical Association (AMA)
Tsuchiya, Ikumi& Hosoya, Takahiro& Ushida, Motoko& Kunimasa, Kazuhiro& Ohta, Toshiro& Kumazawa, Shigenori. Nymphaeol-A Isolated from Okinawan Propolis Suppresses Angiogenesis and Induces Caspase-Dependent Apoptosis via Inactivation of Survival Signals. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-501204
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501204