The Effect of the Combined Action of Roscovitine and Paclitaxel on the Apoptotic and Cell Cycle Regulatory Mechanisms in Colon and Anaplastic Thyroid Cancer Cells
Joint Authors
Tronko, M. D.
Kovzun, O. I.
Pushkarev, V. M.
Pushkarev, V. V.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Aim.
To study the significance of cyclin-dependent kinases (Cdks) in paclitaxel-dependent apoptosis in colon and undifferentiated thyroid cancer cells.
Materials and Methods.
Experiments were performed on undifferentiated thyroid carcinoma (KTC-2) and colon carcinoma (ARO) cell lines.
Cells were treated with paclitaxel (Ptx) and inhibitor of Cdk, roscovitine.
Cell survival test and Western blotting were used for characterization of the effects of paclitaxel and roscovitine on cancer cells.
Results.
It was shown that not c-Jun N-terminal kinase, but cyclin-dependent kinases are responsible for antiapoptotic Bcl-2 phosphorylation.
Cdk inhibition enhanced the cytotoxic effects of Ptx at low drug concentrations.
There was antagonism between Ptx and roscovitine at higher (25 nM) paclitaxel concentrations.
Conclusion.
Using of paclitaxel at low (2.5 to 5 nM) concentrations and roscovitine is a promising combination for further preclinical trials for the development of new therapeutic approaches to the treatment of colon and anaplastic thyroid cancer.
American Psychological Association (APA)
Pushkarev, V. V.& Kovzun, O. I.& Pushkarev, V. M.& Tronko, M. D.. 2012. The Effect of the Combined Action of Roscovitine and Paclitaxel on the Apoptotic and Cell Cycle Regulatory Mechanisms in Colon and Anaplastic Thyroid Cancer Cells. ISRN Biochemistry،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-501214
Modern Language Association (MLA)
Pushkarev, V. V.…[et al.]. The Effect of the Combined Action of Roscovitine and Paclitaxel on the Apoptotic and Cell Cycle Regulatory Mechanisms in Colon and Anaplastic Thyroid Cancer Cells. ISRN Biochemistry No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-501214
American Medical Association (AMA)
Pushkarev, V. V.& Kovzun, O. I.& Pushkarev, V. M.& Tronko, M. D.. The Effect of the Combined Action of Roscovitine and Paclitaxel on the Apoptotic and Cell Cycle Regulatory Mechanisms in Colon and Anaplastic Thyroid Cancer Cells. ISRN Biochemistry. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-501214
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501214