![](/images/graphics-bg.png)
In Vitro Chondrogenesis Transformation Study of Mouse Dental Pulp Stem Cells
Joint Authors
Megat Abdul Wahab, Rohaya
Kermani, Shabnam
Senafi, Sahidan
Zainal Ariffin, Zaidah
Abdul Razak, Mohamad
Zainal Ariffin, Shahrul Hisham
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
A major challenge in the application of mesenchymal stem cells in cartilage reconstruction is that whether the cells are able to differentiate into fully mature chondrocytes before grafting.
The aim of this study was to isolate mouse dental pulp stem cells (DPSC) and differentiate them into chondrocytes.
For this investigation, morphological, molecular, and biochemical analyses for differentiated cells were used.
To induce the chondrocyte differentiation, DPSC were cultured in chondrogenic medium (Zen-Bio, Inc.).
Based on morphological analyses using toluidine blue staining, proteoglycan products appear in DPSC after 21 days of chondrocyte induction.
Biochemical analyses in differentiated group showed that alkaline phosphatase activity was significantly increased at day 14 as compared to control (P<0.05).
Cell viability analyses during the differentiation to chondrocytes also showed that these cells were viable during differentiation.
However, after the 14th day of differentiation, there was a significant decrease (P<0.05) in the viability proportion among differentiated cells as compared to the control cells.
In RT-PCR molecular analyses, mouse DPSC expressed Cd146 and Cd166 which indicated that these cells belong to mesenchymal stem cells.
Coll I and Coll II markers showed high expression after 14 and 21 days, respectively.
In conclusion, this study showed that DPSC successfully differentiated into chondrocytes.
American Psychological Association (APA)
Zainal Ariffin, Shahrul Hisham& Kermani, Shabnam& Megat Abdul Wahab, Rohaya& Senafi, Sahidan& Zainal Ariffin, Zaidah& Abdul Razak, Mohamad. 2012. In Vitro Chondrogenesis Transformation Study of Mouse Dental Pulp Stem Cells. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-501291
Modern Language Association (MLA)
Zainal Ariffin, Shahrul Hisham…[et al.]. In Vitro Chondrogenesis Transformation Study of Mouse Dental Pulp Stem Cells. The Scientific World Journal No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-501291
American Medical Association (AMA)
Zainal Ariffin, Shahrul Hisham& Kermani, Shabnam& Megat Abdul Wahab, Rohaya& Senafi, Sahidan& Zainal Ariffin, Zaidah& Abdul Razak, Mohamad. In Vitro Chondrogenesis Transformation Study of Mouse Dental Pulp Stem Cells. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-501291
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501291