Tumor and Endothelial Cell Hybrids Participate in Glioblastoma Vasculature
Joint Authors
El Houfi, Younas
Idbaih, Ahmed
Labussière, Marianne
Boisselier, Blandine
Thomas, Jean-Léon
Eichmann, Anne
Ravassard, Philippe
Mokhtari, Karima
Delattre, Jean-Yves
El Hallani, Soufiane
Sanson, Marc
Marie, Yannick
Colin, Carole
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Recently antiangiogenic therapy with bevacizumab has shown a high but transient efficacy in glioblastoma (GBM).
Indeed, GBM is one of the most angiogenic human tumors and endothelial proliferation is a hallmark of the disease.
We therefore hypothesized that tumor cells may participate in endothelial proliferation of GBM.
Materials and Methods.
We used EGFR FISH Probe to detect EGFR amplification and anti-CD31, CD105, VE-cadherin, and vWF to identify endothelial cells.
Endothelial and GBM cells were grown separately, labeled with GFP and DsRed lentiviruses, and then cocultured with or without contact.
Results.
In a subset of GBM tissues, we found that several tumor endothelial cells carry EGFR amplification, characteristic of GBM tumor cells.
This observation was reproduced in vitro: when tumor stem cells derived from GBM were grown in the presence of human endothelial cells, a fraction of them acquired endothelial markers (CD31, CD105, VE-cadherin, and vWF).
By transduction with GFP and DsRed expressing lentiviral vectors, we demonstrate that this phenomenon is due to cell fusion and not transdifferentiation.
Conclusion.
A fraction of GBM stem cells thus has the capacity to fuse with endothelial cells and the resulting hybrids may participate in tumor microvascular proliferation and in treatment resistance.
American Psychological Association (APA)
El Hallani, Soufiane& Colin, Carole& El Houfi, Younas& Idbaih, Ahmed& Boisselier, Blandine& Marie, Yannick…[et al.]. 2014. Tumor and Endothelial Cell Hybrids Participate in Glioblastoma Vasculature. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-501316
Modern Language Association (MLA)
El Hallani, Soufiane…[et al.]. Tumor and Endothelial Cell Hybrids Participate in Glioblastoma Vasculature. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-501316
American Medical Association (AMA)
El Hallani, Soufiane& Colin, Carole& El Houfi, Younas& Idbaih, Ahmed& Boisselier, Blandine& Marie, Yannick…[et al.]. Tumor and Endothelial Cell Hybrids Participate in Glioblastoma Vasculature. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-501316
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501316