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P2Y Receptor Modulation of ATP Release in the Urothelium
Joint Authors
Mansfield, Kylie J.
Hughes, Jessica R.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The release of ATP from the urothelium in response to stretch during filling demonstrates the importance of the purinergic system for the physiological functioning of the bladder.
This study examined the effect of P2 receptor agonists on ATP release from two urothelial cell lines (RT4 and UROtsa cells).
Hypotonic Krebs was used as a stretch stimulus.
Incubation of urothelial cells with high concentrations of the P2Y agonist ADP induced ATP release to a level that was 40-fold greater than hypotonic-stimulated ATP release (P < 0.0011, ADP EC50 1.8 µM).
Similarly, an increase in ATP release was also observed with the P2Y agonist, UTP, up to a maximum of 70% of the hypotonic response (EC50 0.62 µM).
Selective P2 receptor agonists, αβ-methylene-ATP, ATP-γ-S, and 2-methylthio-ADP had minimal effects on ATP release.
ADP-stimulated ATP release was significantly inhibited by suramin (100 µM, P = 0.002).
RT4 urothelial cells break down nucleotides (100 µM) including ATP, ADP, and UTP to liberate phosphate.
Phosphate liberation was also demonstrated from endogenous nucleotides with approximately 10% of the released ATP broken down during the incubation.
These studies demonstrate a role for P2Y receptor activation in stimulation of ATP release and emphasize the complexity of urothelial P2 receptor signalling.
American Psychological Association (APA)
Mansfield, Kylie J.& Hughes, Jessica R.. 2014. P2Y Receptor Modulation of ATP Release in the Urothelium. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-501555
Modern Language Association (MLA)
Mansfield, Kylie J.& Hughes, Jessica R.. P2Y Receptor Modulation of ATP Release in the Urothelium. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-501555
American Medical Association (AMA)
Mansfield, Kylie J.& Hughes, Jessica R.. P2Y Receptor Modulation of ATP Release in the Urothelium. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-501555
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501555