![](/images/graphics-bg.png)
Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3
Joint Authors
Gu, Shao-Hua
Shi, Hui-Juan
Sun, Xiao-Xi
Tang, Hai-Ping
Xie, Yi
He, Ya-Ping
Wang, Jian
Ji, Chao-Neng
Xu, Wan-Xiang
Source
Clinical and Developmental Immunology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The human zona pellucida glycoprotein-3 (hZP3) by virtue of its critical role during fertilization has been proposed as a promising candidate antigen to develop a contraceptive vaccine.
In this direction, it is imperative to map minimal motifs of the B cell epitopes (BCEs) so as to avoid ZP-specific oophoritogenic T cell epitopes (TCEs) in the ZP3-based immunogens.
In this study, based on known results of mapping marmoset and bonnet monkey ZP3 (mstZP3 and bmZP3), two predictable epitopes23–30 and 301–320 on hZP3 were first confirmed and five minimal motifs within four epitopes on hZP3 were defined using serum to recombinant hZP3a22–176 or hZP3b177–348 as well as a biosynthetic peptide strategy.
These defined minimal motifs were QPLWLL23–28 for hZP323–30, MQVTDD103–108 for hZP393–110, EENW178–181 for hZP3172–190, as well as SNSWF306–310 and EGP313–315 for hZP3301–320, respectively.
Furthermore, the antigenicity of two peptides for hZP3172–187 and hZP3301–315 and specificity of the antibody response to these peptides were also evaluated, which produced high-titer antibodies in immunized animals that were capable of reacting to ZP on human oocytes, r-hZP3b177–348 protein, as well as r-hZP3172–190, r-hZP3303–310, and r-hZP3313–320 epitope peptides fused with truncated GST188 protein.
American Psychological Association (APA)
Xu, Wan-Xiang& He, Ya-Ping& Wang, Jian& Tang, Hai-Ping& Shi, Hui-Juan& Sun, Xiao-Xi…[et al.]. 2011. Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3. Clinical and Developmental Immunology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-501616
Modern Language Association (MLA)
Xu, Wan-Xiang…[et al.]. Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3. Clinical and Developmental Immunology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-501616
American Medical Association (AMA)
Xu, Wan-Xiang& He, Ya-Ping& Wang, Jian& Tang, Hai-Ping& Shi, Hui-Juan& Sun, Xiao-Xi…[et al.]. Mapping of Minimal Motifs of B-Cell Epitopes on Human Zona Pellucida Glycoprotein-3. Clinical and Developmental Immunology. 2011. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-501616
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501616