Stem Cells Grown in Osteogenic Medium on PLGA, PLGAHA, and Titanium Scaffolds for Surgical Applications
Joint Authors
Dorati, Rossella
Benazzo, Francesco
Asti, Annalia
Saino, Enrica
Visai, Livia
Gastaldi, Giulia
Conti, Bice
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-23
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Pluripotent adipose tissue-derived stem cells (hASCs) can differentiate into various mesodermal cell types such as osteoblasts, chondroblasts, and myoblasts.
We isolated hASCs from subcutaneous adipose tissue during orthopaedic surgery and induced the osteogenic differentiation for 28 days on three different synthetic scaffolds such as polylactide-co-glycolide (PLGA), polylactide-co-glycolide/hydroxyapatite (PLGA/HA), and trabecular titanium scaffolds (Ti6Al4V).
Pore size can influence certain criteria such as cell attachment, infiltration, and vascularization.
The aim of this study was to investigate the performance of PLGA and PLGA/HA scaffolds with a higher porosity, ranging between 75% and 84%, with respect to Ti scaffolds but with smaller pore size, seeded with hASCs to develop a model that could be used in the treatment of bone defects and fractures.
Osteogenesis was assessed by ELISA quantitation of extracellular matrix protein expression, von Kossa staining, X-ray microanalysis, and scanning electron microscopy.
The higher amount of protein matrix on the Ti scaffold with respect to PLGA and PLGA/HA leads to the conclusion that not only the type of material but the structure significantly affects cell proliferation.
American Psychological Association (APA)
Asti, Annalia& Gastaldi, Giulia& Dorati, Rossella& Saino, Enrica& Conti, Bice& Visai, Livia…[et al.]. 2010. Stem Cells Grown in Osteogenic Medium on PLGA, PLGAHA, and Titanium Scaffolds for Surgical Applications. Bioinorganic Chemistry and Applications،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-501620
Modern Language Association (MLA)
Asti, Annalia…[et al.]. Stem Cells Grown in Osteogenic Medium on PLGA, PLGAHA, and Titanium Scaffolds for Surgical Applications. Bioinorganic Chemistry and Applications No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-501620
American Medical Association (AMA)
Asti, Annalia& Gastaldi, Giulia& Dorati, Rossella& Saino, Enrica& Conti, Bice& Visai, Livia…[et al.]. Stem Cells Grown in Osteogenic Medium on PLGA, PLGAHA, and Titanium Scaffolds for Surgical Applications. Bioinorganic Chemistry and Applications. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-501620
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501620