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The PCR-Based Diagnosis of Central Nervous System Tuberculosis : Up to Date
Joint Authors
Takasu, Toshiaki
Takahashi, Teruyuki
Tamura, Masato
Source
Tuberculosis Research and Treatment
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-13
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Central nervous system (CNS) tuberculosis, particularly tuberculous meningitis (TBM), is the severest form of Mycobacterium tuberculosis (M.Tb) infection, causing death or severe neurological defects in more than half of those affected, in spite of recent advancements in available anti-tuberculosis treatment.
The definitive diagnosis of CNS tuberculosis depends upon the detection of M.Tb bacilli in the cerebrospinal fluid (CSF).
At present, the diagnosis of CNS tuberculosis remains a complex issue because the most widely used conventional “gold standard” based on bacteriological detection methods, such as direct smear and culture identification, cannot rapidly detect M.Tb in CSF specimens with sufficient sensitivity in the acute phase of TBM.
Recently, instead of the conventional “gold standard”, the various molecular-based methods including nucleic acid amplification (NAA) assay technique, particularly polymerase chain reaction (PCR) assay, has emerged as a promising new method for the diagnosis of CNS tuberculosis because of its rapidity, sensitivity and specificity.
In addition, the innovation of nested PCR assay technique is worthy of note given its contribution to improve the diagnosis of CNS tuberculosis.
In this review, an overview of recent progress of the NAA methods, mainly highlighting the PCR assay technique, was presented.
American Psychological Association (APA)
Takahashi, Teruyuki& Tamura, Masato& Takasu, Toshiaki. 2012. The PCR-Based Diagnosis of Central Nervous System Tuberculosis : Up to Date. Tuberculosis Research and Treatment،Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-501639
Modern Language Association (MLA)
Takahashi, Teruyuki…[et al.]. The PCR-Based Diagnosis of Central Nervous System Tuberculosis : Up to Date. Tuberculosis Research and Treatment No. 2012 (2012), pp.1-17.
https://search.emarefa.net/detail/BIM-501639
American Medical Association (AMA)
Takahashi, Teruyuki& Tamura, Masato& Takasu, Toshiaki. The PCR-Based Diagnosis of Central Nervous System Tuberculosis : Up to Date. Tuberculosis Research and Treatment. 2012. Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-501639
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501639