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Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1
Joint Authors
Abdeshahian, Peyman
Abdul Hamid, Aidil
Wan Yusoff, Wan Mohtar
Kalil, Mohd Sahaid
Ganjali Dashti, Marjan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The biosynthesis of biomedical products including lipid and gamma-linolenic acid (GLA) by Cunninghamella bainieri 2A1 was studied in repeated batch fermentation.
Three key process variables, namely, glucose concentration, ammonium tartrate concentration, and harvesting time, were optimized using response surface methodology.
Repeated batch fermentation was carried out by the cultivation of Cunninghamella bainieri 2A1 in nitrogen-limited medium with various nitrogen concentration (1–4 g/L) and glucose concentration (20–40 g/L) at three time intervals (12 h, 24 h, and 48 h).
Experimental results showed that the highest lipid concentration of 6.2 g/L and the highest GLA concentration of 0.4 g/L were obtained in optimum conditions, where 20.2 g/L glucose, 2.12 g/L ammonium tartrate, and 48 h harvesting time were utilized.
Statistical results showed that the interaction between glucose and ammonium tartrate concentration had highly significant effects on lipid and GLA biosynthesis (P<0.01).
Moreover, harvesting time had a significant interaction effect with glucose and ammonium tartrate concentration on lipid production (P<0.05).
American Psychological Association (APA)
Ganjali Dashti, Marjan& Abdeshahian, Peyman& Wan Yusoff, Wan Mohtar& Kalil, Mohd Sahaid& Abdul Hamid, Aidil. 2014. Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-501695
Modern Language Association (MLA)
Ganjali Dashti, Marjan…[et al.]. Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-501695
American Medical Association (AMA)
Ganjali Dashti, Marjan& Abdeshahian, Peyman& Wan Yusoff, Wan Mohtar& Kalil, Mohd Sahaid& Abdul Hamid, Aidil. Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-501695
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501695