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Proteomic and Bioinformatics Analyses of Mouse Liver Microsomes
Joint Authors
Zhan, Xianquan
Fang, Fan
Li, Mao-Yu
Peng, Fang
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-24, 24 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-20
Country of Publication
Egypt
No. of Pages
24
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Microsomes are derived mostly from endoplasmic reticulum and are an ideal target to investigate compound metabolism, membrane-bound enzyme functions, lipid-protein interactions, and drug-drug interactions.
To better understand the molecular mechanisms of the liver and its diseases, mouse liver microsomes were isolated and enriched with differential centrifugation and sucrose gradient centrifugation, and microsome membrane proteins were further extracted from isolated microsomal fractions by the carbonate method.
The enriched microsome proteins were arrayed with two-dimensional gel electrophoresis (2DE) and carbonate-extracted microsome membrane proteins with one-dimensional gel electrophoresis (1DE).
A total of 183 2DE-arrayed proteins and 99 1DE-separated proteins were identified with tandem mass spectrometry.
A total of 259 nonredundant microsomal proteins were obtained and represent the proteomic profile of mouse liver microsomes, including 62 definite microsome membrane proteins.
The comprehensive bioinformatics analyses revealed the functional categories of those microsome proteins and provided clues into biological functions of the liver.
The systematic analyses of the proteomic profile of mouse liver microsomes not only reveal essential, valuable information about the biological function of the liver, but they also provide important reference data to analyze liver disease-related microsome proteins for biomarker discovery and mechanism clarification of liver disease.
American Psychological Association (APA)
Peng, Fang& Zhan, Xianquan& Li, Mao-Yu& Fang, Fan. 2012. Proteomic and Bioinformatics Analyses of Mouse Liver Microsomes. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-24.
https://search.emarefa.net/detail/BIM-501763
Modern Language Association (MLA)
Peng, Fang…[et al.]. Proteomic and Bioinformatics Analyses of Mouse Liver Microsomes. International Journal of Proteomics No. 2012 (2012), pp.1-24.
https://search.emarefa.net/detail/BIM-501763
American Medical Association (AMA)
Peng, Fang& Zhan, Xianquan& Li, Mao-Yu& Fang, Fan. Proteomic and Bioinformatics Analyses of Mouse Liver Microsomes. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-24.
https://search.emarefa.net/detail/BIM-501763
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501763