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Assessment of Status of rpoB Gene in FNAC Samples of Tuberculous Lymphadenitis by Real-Time PCR
Joint Authors
Source
Tuberculosis Research and Treatment
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-15
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Introduction.
Multidrug resistance tuberculosis (MDR TB), the combined resistance of Mycobacterium tuberculosis to isoniazid (INH) and rifampin (RFM) is a major public health problem in India as it ranks second among the MDR-TB high burden countries worldwide.
WHO recommends RFM resistance as a “surrogate marker” for detecting MDR.
FNAC is the most widely used noninvasive investigative technique for TB lymphadenitis.
Real-time polymerase chain reaction, an extremely versatile technique can be used for the timely detection and treatment of MDR TB by assessing RFM resistance status in the FNAC samples of TB lymphadenitis.
Aim.
To assess the status of rpoB gene by real-time PCR in FNAC samples of TB lymphadenitis.
Materials and Methods.
Thirty FNAC samples from patients with persistent LAP or appearance of new LAP after 5 months or more of Anti Tubercular Treatment were assessed for status of rpoB gene by Real-Time PCR using probe covering the “hot spot resistance” region of the rpoB gene.
Result.
By using probe covering codons 531 and 526 of rpoB gene, we could detect 17 of 30 (56.7%) rifampin resistant isolate.
The PCR could detect Mtb DNA in 100% of cases.
Conclusion.
Use of molecular methods like Real-Time PCR for detection of MDR-TB in FNAC samples is time saving, logical and economical approach over the culture based method.
American Psychological Association (APA)
Raoot, Amita& Dev, Geeta. 2012. Assessment of Status of rpoB Gene in FNAC Samples of Tuberculous Lymphadenitis by Real-Time PCR. Tuberculosis Research and Treatment،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-501874
Modern Language Association (MLA)
Raoot, Amita& Dev, Geeta. Assessment of Status of rpoB Gene in FNAC Samples of Tuberculous Lymphadenitis by Real-Time PCR. Tuberculosis Research and Treatment No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-501874
American Medical Association (AMA)
Raoot, Amita& Dev, Geeta. Assessment of Status of rpoB Gene in FNAC Samples of Tuberculous Lymphadenitis by Real-Time PCR. Tuberculosis Research and Treatment. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-501874
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501874