In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang
Joint Authors
Nolé, Tsabang
Freitas-Junior, Lucio
Ayong, Lawrence S.
Vivarelli, Diego
Guantai, Anastasia Nkatha
Arrey Tarkang, Protus
Agbor, Gabriel Agbor
Liuzzi, Michel
Franzoi, Kathrin Diehl
Lee, Sukjun
Okalebo, Faith Apoelot
Lee, Eunyoung
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Nefang, a polyherbal product composed of Mangifera indica (bark and leaf), Psidium guajava, Carica papaya, Cymbopogon citratus, Citrus sinensis, and Ocimum gratissimum (leaves), is a potential therapy against P.
falciparum malaria.
In vitro antiplasmodial activities of its constituent solvent extracts were analyzed on CQ-sensitive (3D7) and multidrug resistant (Dd2) P.
falciparum strains.
The interactions involving the differential solvent extracts were further analyzed using a variable potency ratio drug combination approach.
Effective concentration 50 (EC50) values were determined by nonlinear regression curve-fitting of the dose-response data and used in calculating the fractional inhibitory concentration 50 (FIC50) and combination indices (CI) for each pair.
The derived EC50 values (3D7/Dd2, μg/mL) are Nefang-96.96/55.08, MiB-65.33/34.58, MiL-82.56/40.04, Pg-47.02/25.79, Cp-1188/317.5, Cc-723.3/141, Cs-184.4/105.1, and Og-778.5/118.9.
Synergism was obtained with MiB/Pg (CI = 0.351), MiL/Pg (0.358), MiB/Cs (0.366), MiL/Cs (0.482), Pg/Cs (0.483), and Cs/Og (0.414) when analyzed at equipotency ratios.
Cytotoxicity testing of Nefang and the solvent extracts on two human cell lines (Hep G2 and U2OS) revealed no significant toxicity relative to their antiplasmodial activities (SI > 20).
Taken together, our data confirm the antimalarial activities of Nefang and its constituent plant extracts and identified extract pairs with promising synergistic interactions for exploitation towards a rational phytotherapeutic and evidence-based antimalarial drug discovery.
American Psychological Association (APA)
Arrey Tarkang, Protus& Franzoi, Kathrin Diehl& Lee, Sukjun& Lee, Eunyoung& Vivarelli, Diego& Freitas-Junior, Lucio…[et al.]. 2014. In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-501888
Modern Language Association (MLA)
Arrey Tarkang, Protus…[et al.]. In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-501888
American Medical Association (AMA)
Arrey Tarkang, Protus& Franzoi, Kathrin Diehl& Lee, Sukjun& Lee, Eunyoung& Vivarelli, Diego& Freitas-Junior, Lucio…[et al.]. In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-501888
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501888