Expression of the Lactate Dehydrogenase Gene from Eptatretus okinoseanus in Escherichia coli
Joint Authors
Nishiguchi, Yoshikazu
Oshima, Noriko
Uchida, Akira
Okada, Mitsumasa
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-30
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Amplified Eptatretus okinoseanus cDNA was digested with NdeI and EcoRI, cloned into pCold trigger factor (TF), and transformed with Escherichia coli strain BL 21 in which a csp A promoter was introduced to inhibit the expression of foreign peptides.
Recombinant lactate dehydrogenase (LDH) was obtained in the soluble fraction after sonication of the cells.
The protein was digested by HRC 3C protease, thrombin, and factor Xa.
The specific activity of TF-tagged protein and tagless protein were 0.646×106 mIU/mg and 3.56×106 mIU/mg, respectively.
The deletion of the TF tag enhanced the activity compared with the native protein to 13.4×106 mIU/mg, showing that this expression method is effective for the mass production of the protein to allow further study of the structure of LDH.
American Psychological Association (APA)
Nishiguchi, Yoshikazu& Uchida, Akira& Oshima, Noriko& Okada, Mitsumasa. 2011. Expression of the Lactate Dehydrogenase Gene from Eptatretus okinoseanus in Escherichia coli. ISRN Zoology،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-501908
Modern Language Association (MLA)
Nishiguchi, Yoshikazu…[et al.]. Expression of the Lactate Dehydrogenase Gene from Eptatretus okinoseanus in Escherichia coli. ISRN Zoology No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-501908
American Medical Association (AMA)
Nishiguchi, Yoshikazu& Uchida, Akira& Oshima, Noriko& Okada, Mitsumasa. Expression of the Lactate Dehydrogenase Gene from Eptatretus okinoseanus in Escherichia coli. ISRN Zoology. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-501908
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501908