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Collagen Extraction from Malaysian Cultured Catfish (Hybrid Clarias Sp.) : Kinetics and Optimization of Extraction Conditions Using Response Surface Methodology
Joint Authors
Kiew, Peck Loo
Mat Don, Mashitah
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Engineering Sciences and Information Technology
Abstract EN
A central composite design (CCD) was used for the experimental design and results analysis to obtain the optimal processing parameters (acetic acid concentration, liquid to solid ratio, and stirring speed) for the extraction of pepsin soluble collagen (PSC) from muscles of cultured hybrid catfish of Clarias sp.
(Clarias gariepinus × C.
macrocephalus).
Statistical analysis showed that the linear and quadratic terms of these three independent variables had significant effects on the yield of PSC.
There was also an interaction between the ratio of liquid to solid and the stirring speed in affecting the extraction efficiency.
Optimal conditions for a higher yield of PSC were an acetic acid concentration of 0.67 M, a liquid to solid ratio of 24.65 ml/g, and the stirring speed of 423.64 rpm.
The verification of the optimization showed that the percentage error differences between the experimental and predicted values were in the range of 0.22–4.42%.
The experimental values agreed with the predicted values, indicating an excellent fit of the model used and the success of the response surface methodology in modeling the extraction of PSC from the muscles of catfish.
The experimental results were also fitted to the power law model and it was proven to be appropriate in describing the kinetics of collagen extraction process.
American Psychological Association (APA)
Kiew, Peck Loo& Mat Don, Mashitah. 2012. Collagen Extraction from Malaysian Cultured Catfish (Hybrid Clarias Sp.) : Kinetics and Optimization of Extraction Conditions Using Response Surface Methodology. ISRN Chemical Engineering،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-501922
Modern Language Association (MLA)
Kiew, Peck Loo& Mat Don, Mashitah. Collagen Extraction from Malaysian Cultured Catfish (Hybrid Clarias Sp.) : Kinetics and Optimization of Extraction Conditions Using Response Surface Methodology. ISRN Chemical Engineering No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-501922
American Medical Association (AMA)
Kiew, Peck Loo& Mat Don, Mashitah. Collagen Extraction from Malaysian Cultured Catfish (Hybrid Clarias Sp.) : Kinetics and Optimization of Extraction Conditions Using Response Surface Methodology. ISRN Chemical Engineering. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-501922
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501922