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Agrobacterium-Mediated Gene Transfer to Cereal Crop Plants : Current Protocols for Barley, Wheat, Triticale, and Maize
Joint Authors
Hensel, Goetz
Riechen, Jan
Oleszczuk, Sylwia
Kumlehn, Jochen
Kastner, Christine
Source
International Journal of Plant Genomics
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-06-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The development of powerful “omics” technologies has enabled researchers to identify many genes of interest for which comprehensive functional analyses are highly desirable.
However, the production of lines which ectopically express recombinant genes, or those in which endogenous genes are knocked down via stable transformation, remains a major bottleneck for the association between genetics and gene function in monocotyledonous crops.
Methods of effective DNA transfer into regenerable cells of immature embryos from cereals by means of Agrobacterium tumefaciens have been modified in a stepwise manner.
The effect of particular improvement measures has often not been significantly evident, whereas their combined implementation has resulted in meaningful advances.
Here, we provide updated protocols for the Agrobacterium-mediated generation of stably transgenic barley, wheat, triticale and maize.
Based upon these methods, several hundred independent transgenic lines have been delivered, with efficiencies of inoculated embryos leading to stably transgenic plants reaching 86% in barley, 10% in wheat, 4% in triticale, and 24% in maize.
American Psychological Association (APA)
Hensel, Goetz& Kastner, Christine& Oleszczuk, Sylwia& Riechen, Jan& Kumlehn, Jochen. 2009. Agrobacterium-Mediated Gene Transfer to Cereal Crop Plants : Current Protocols for Barley, Wheat, Triticale, and Maize. International Journal of Plant Genomics،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-501933
Modern Language Association (MLA)
Hensel, Goetz…[et al.]. Agrobacterium-Mediated Gene Transfer to Cereal Crop Plants : Current Protocols for Barley, Wheat, Triticale, and Maize. International Journal of Plant Genomics No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-501933
American Medical Association (AMA)
Hensel, Goetz& Kastner, Christine& Oleszczuk, Sylwia& Riechen, Jan& Kumlehn, Jochen. Agrobacterium-Mediated Gene Transfer to Cereal Crop Plants : Current Protocols for Barley, Wheat, Triticale, and Maize. International Journal of Plant Genomics. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-501933
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501933