Ultraviolet Spectrophotometric Method for Determination of Glipizide in Presence of LiposomalProliposomal Turbidity
Joint Authors
Nagori, Badri Prakash
Issarani, Roshan
Prasad, Neelkant
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-11
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A simple and sensitive ultraviolet spectrophotometric method for quantitative estimation of glipizide in presence of lipid turbidity is described to avoid false estimation due to diffraction by turbidity.
UV detection was performed at 230 nm, 225 nm, and 235 nm, and the calibration curve was plotted between resultant of absorbance of [230 nm − (225 nm + 235 nm)/2] and concentration of analyte.
The calibration curve was linear over the concentration range tested (1–20 μg/mL) with limit of detection of 0.27 μg/mL and limit of quantification of 0.82 μg/mL.
Percent relative standard deviations and percent relative mean error, representing precision and accuracy, respectively, for clear as well as turbid solutions, were found to be within acceptable limits, that is, always less than 0.69 and 0.41, respectively, for clear solution and 0.65 and 0.47, respectively, for turbid solution.
Conclusively, our method was successfully applied for the determination of glipizide in clear as well as turbid solutions, and it was found that the drug analyte in both types of solutions can be detected from the same calibration curve accurately and precisely and glipizide entrapped in the liposomes or in proliposomal matrix was not detected.
American Psychological Association (APA)
Prasad, Neelkant& Issarani, Roshan& Nagori, Badri Prakash. 2013. Ultraviolet Spectrophotometric Method for Determination of Glipizide in Presence of LiposomalProliposomal Turbidity. Journal of Spectroscopy،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-502010
Modern Language Association (MLA)
Prasad, Neelkant…[et al.]. Ultraviolet Spectrophotometric Method for Determination of Glipizide in Presence of LiposomalProliposomal Turbidity. Journal of Spectroscopy No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-502010
American Medical Association (AMA)
Prasad, Neelkant& Issarani, Roshan& Nagori, Badri Prakash. Ultraviolet Spectrophotometric Method for Determination of Glipizide in Presence of LiposomalProliposomal Turbidity. Journal of Spectroscopy. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-502010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502010