Evidence for the Existence of Triple-Negative Variants in the MCF-7 Breast Cancer Cell Population
Joint Authors
Kim, Ji Eun
Baguley, Bruce C.
Leung, Euphemia
Askarian-Amiri, Marjan
Finlay, Graeme J.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The MCF-7 line, derived in 1973 from a malignant pleural effusion, is one of the most commonly used culture models for human breast cancer.
Despite its long history, MCF-7 is a surprisingly heterogeneous line.
We previously showed that if MCF-7 cells were cultured for a prolonged period either in the absence of estrogen or in the presence of the antiestrogen tamoxifen, sub-lines were selected that differed from the parental line in ploidy, mean cell volume, signaling pathway usage, and drug sensitivity.
This suggests a process of selection of preexisting variants rather than of adaptation of the parental line.
All the sublines were estrogen receptor (ER) positive, raising the question of whether MCF-7 also contains ER negative variants.
Here, we have looked for such variants by culturing for a prolonged period in the presence of fulvestrant, an estrogen antagonist that has no estrogen agonist activity.
Three sublines were developed, each of which was ER negative, progesterone receptor (PR) negative and expressed only a low level of HER2.
Each of the variants differed from the original MCF-7 line in ploidy, modal cell volume, and signaling pathway usage.
Control experiments in which cells were cultured for a prolonged period in the absence of estrogen selected for variants that were ER and PR positive.
The properties of the triple-negative MCF-7 were compared with those of an existing triple-negative cell line, MDA-MB-231, and human epidermal growth factor receptor 2 (HER2)+ SKBr3, as well as from those of the “immortalized” breast epithelial line MCF10A.
The results suggest that new variants or phenotypes of MCF-7 might be generated continuously in culture, and by implication this might apply to breast cancer development and even normal breast epithelial development in vivo.
American Psychological Association (APA)
Leung, Euphemia& Kim, Ji Eun& Askarian-Amiri, Marjan& Finlay, Graeme J.& Baguley, Bruce C.. 2014. Evidence for the Existence of Triple-Negative Variants in the MCF-7 Breast Cancer Cell Population. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-502044
Modern Language Association (MLA)
Leung, Euphemia…[et al.]. Evidence for the Existence of Triple-Negative Variants in the MCF-7 Breast Cancer Cell Population. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-502044
American Medical Association (AMA)
Leung, Euphemia& Kim, Ji Eun& Askarian-Amiri, Marjan& Finlay, Graeme J.& Baguley, Bruce C.. Evidence for the Existence of Triple-Negative Variants in the MCF-7 Breast Cancer Cell Population. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-502044
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502044