Contrasting Effects of Systemic MonocyteMacrophage and CD4+ T Cell Depletion in a Reversible Ureteral Obstruction Mouse Model of Chronic Kidney Disease
Joint Authors
Quigg, Richard J.
Shakaib, Mohammed
Puri, Tipu S.
Chaves, Lee D.
Chang, Anthony
Mathew, Liby
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Using a reversible UUO model (rUUO), we have demonstrated that C57BL/6 mice are susceptible to development of CKD after obstruction-mediated kidney injury while BALB/c mice are resistant.
We hypothesized that selective systemic depletion of subpopulations of inflammatory cells during injury or repair might alter the development of CKD.
To investigate the impact of modification of Th-lymphocytes or macrophage responses on development of CKD after rUUO, we used an anti-CD4 antibody (GK1.5) or liposomal clodronate to systemically deplete CD4+ T cells or monocyte/macrophages, respectively, prior to and throughout the rUUO protocol.
Flow cytometry and immunohistochemistry confirmed depletion of target cell populations.
C57BL/6 mice treated with the GK1.5 antibody to deplete CD4+ T cells had higher BUN levels and delayed recovery from rUUO.
Treatment of C57BL/6 mice with liposomal clodronate to deplete monocyte/macrophages led to a relative protection from CKD as assessed by BUN values.
Our results demonstrate that modulation of the inflammatory response during injury and repair altered the susceptibility of C57BL/6 mice to development of CKD in our rUUO model.
American Psychological Association (APA)
Chaves, Lee D.& Mathew, Liby& Shakaib, Mohammed& Chang, Anthony& Quigg, Richard J.& Puri, Tipu S.. 2013. Contrasting Effects of Systemic MonocyteMacrophage and CD4+ T Cell Depletion in a Reversible Ureteral Obstruction Mouse Model of Chronic Kidney Disease. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-502056
Modern Language Association (MLA)
Chaves, Lee D.…[et al.]. Contrasting Effects of Systemic MonocyteMacrophage and CD4+ T Cell Depletion in a Reversible Ureteral Obstruction Mouse Model of Chronic Kidney Disease. Clinical and Developmental Immunology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-502056
American Medical Association (AMA)
Chaves, Lee D.& Mathew, Liby& Shakaib, Mohammed& Chang, Anthony& Quigg, Richard J.& Puri, Tipu S.. Contrasting Effects of Systemic MonocyteMacrophage and CD4+ T Cell Depletion in a Reversible Ureteral Obstruction Mouse Model of Chronic Kidney Disease. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-502056
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502056