Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars
Joint Authors
Sané, Djibril
Guèye, Badara
Daher, Abdourahman
Duval, Yves
Borgel, Alain
Diatta, Léopold Ibrahima Djitiningo
Aberlenc-Bertossi, Frédérique
Sagna, Maurice
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
This study provides a physiological analysis of somatic embryogenesis in four elite cultivars of date palms: Ahmar, Amsekhsi, Tijib, and Amaside, from the initial callogenesis to establishment and proliferation of embryogenic suspension cultures.
Somatic embryos development and in vitro plants rooting were also studied.
For each step, auxins and cytokinins concentrations were optimised.
The primary callogenesis from leaf explants of seedlings appeared highly dependent on genotype.
Ahmar (80%) and Amsekhsi (76%) appeared highly callogenic, whereas Tijib (10%) and Amaside (2%) produced low amounts of calluses.
2,4-Dichlorophenoxyacetic acid appeared favorable to the induction of primary callogenesis and its effect was enhanced by the addition of benzyl adenine or adenine sulfate.
Secondary friable calli obtained from chopped granular calli were used to initiate embryogenic cell suspensions in media supplied with 2,4-dichlorophenoxyacetic acid.
Suspension cultures showed a growth rate of fourfold after four subcultures in presence of 2,4-dichlorophenoxyacetic acid 2 mg/L.
Our results showed that a seven-day transitory treatment with benzyl adenine 0,5 mg/L was necessary to optimize embryos development.
Naphthalene acetic acid induced the development of primary orthogravitropic roots during embryos germination.
The comparison with cytofluorometry of nuclear DNA amounts showed no significant difference in ploidy level between regenerated plants and seedlings.
American Psychological Association (APA)
Sané, Djibril& Aberlenc-Bertossi, Frédérique& Diatta, Léopold Ibrahima Djitiningo& Guèye, Badara& Daher, Abdourahman& Sagna, Maurice…[et al.]. 2012. Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-502110
Modern Language Association (MLA)
Sané, Djibril…[et al.]. Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-502110
American Medical Association (AMA)
Sané, Djibril& Aberlenc-Bertossi, Frédérique& Diatta, Léopold Ibrahima Djitiningo& Guèye, Badara& Daher, Abdourahman& Sagna, Maurice…[et al.]. Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-502110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502110