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Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1−-hVEGF165 by Ultrasound-Targeted Microbubble Destruction
Joint Authors
Zhang, Hong
Gao, Feng
Xie, Xiaojie
Xu, Qiyuan
You, Xiangdong
Pu, Zhaoxia
Jian'an, Wang
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-19
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Ultrasound-targeted microbubble destruction (UTMD) has been proposed as a new technique for organ-specific gene transfer and drug delivery.
This study was performed to investigate the effect of UTMD on marrow mesenchymal stem cells (MSCs) transfected with pcDNA3.1−-hVEGF165.pcDNA3.1−-hVEGF165 were transfected into the third passage of MSCs, with or without UTMD under different ultrasound conditions.
Protein expression was quantified by hVEGF165-ELISA kit after transfection for 24, 48, and 72 hours.
UTMD-mediated transfection of MSCs yielded a significant protein expression.
UTMD of mechanic index (MI) 0.6 for 90 seconds led to the highest level of protein expression.
American Psychological Association (APA)
Pu, Zhaoxia& You, Xiangdong& Xu, Qiyuan& Gao, Feng& Xie, Xiaojie& Zhang, Hong…[et al.]. 2011. Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1−-hVEGF165 by Ultrasound-Targeted Microbubble Destruction. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-502323
Modern Language Association (MLA)
Pu, Zhaoxia…[et al.]. Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1−-hVEGF165 by Ultrasound-Targeted Microbubble Destruction. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-4.
https://search.emarefa.net/detail/BIM-502323
American Medical Association (AMA)
Pu, Zhaoxia& You, Xiangdong& Xu, Qiyuan& Gao, Feng& Xie, Xiaojie& Zhang, Hong…[et al.]. Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1−-hVEGF165 by Ultrasound-Targeted Microbubble Destruction. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-502323
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502323