Preparation and Characterization of a Novel Skin Substitute
Joint Authors
Stella, Maurizio
Castagnoli, Carlotta
Casarin, Stefania
Ostorero, Alessia
Pezzuto, Carla
Casimiri, Raffaella
Cambieri, Irene
Fumagalli, Mara
Alotto, Daniela
Germano, Patrizia
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Autologous epidermal cell cultures (CEA) represent a possibility to treat extensive burn lesions, since they allow a significative surface expansion which cannot be achieved with other surgical techniques based on autologous grafting.
Moreover currently available CEA preparations are difficult to handle and their take rate is unpredictable.
This study aimed at producing and evaluating a new cutaneous biosubstitute made up of alloplastic acellular glycerolized dermis (AAGD) and CEA to overcome these difficulties.
A procedure that maintained an intact basement membrane was developed, so as to promote adhesion and growth of CEA on AAGD.
Keratinocytes were seeded onto AAGD and cultured up to 21 days.
Viability tests and immunohistochemical analysis with specific markers were carried out at 7, 14, and 21 days, to evaluate keratinocyte adhesion, growth, and maturation.
Our results support the hypothesis that this newly formed skin substitute could allow its permanent engraftment in clinical application.
American Psychological Association (APA)
Castagnoli, Carlotta& Fumagalli, Mara& Alotto, Daniela& Cambieri, Irene& Casarin, Stefania& Ostorero, Alessia…[et al.]. 2010. Preparation and Characterization of a Novel Skin Substitute. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-502380
Modern Language Association (MLA)
Castagnoli, Carlotta…[et al.]. Preparation and Characterization of a Novel Skin Substitute. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-502380
American Medical Association (AMA)
Castagnoli, Carlotta& Fumagalli, Mara& Alotto, Daniela& Cambieri, Irene& Casarin, Stefania& Ostorero, Alessia…[et al.]. Preparation and Characterization of a Novel Skin Substitute. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-502380
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502380