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Thrombin-Binding Aptamer Quadruplex Formation : AFM and Voltammetric Characterization
Joint Authors
Chiorcea-Paquim, Ana-Maria
Diculescu, Victor Constantin
Oliveira-Brett, Ana Maria
Eritja, Ramon
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The adsorption and the redox behaviour of thrombin-binding aptamer (TBA) and extended TBA (eTBA) were studied using atomic force microscopy and voltammetry at highly oriented pyrolytic graphite and glassy carbon.
The different adsorption patterns and degree of surface coverage were correlated with the sequence base composition, presence/absence of K+, and voltammetric behaviour of TBA and eTBA.
In the presence of K+, only a few single-stranded sequences present adsorption, while the majority of the molecules forms stable and rigid quadruplexes with no adsorption.
Both TBA and eTBA are oxidized and the only anodic peak corresponds to guanine oxidation.
Upon addition of K+ ions, TBA and eTBA fold into a quadruplex, causing the decrease of guanine oxidation peak and occurrence of a new peak at a higher potential due to the oxidation of G-quartets.
The higher oxidation potential of G-quartets is due to the greater difficulty of electron transfer from the inside of the quadruplex to the electrode surface than electron transfer from the more flexible single strands.
American Psychological Association (APA)
Diculescu, Victor Constantin& Chiorcea-Paquim, Ana-Maria& Eritja, Ramon& Oliveira-Brett, Ana Maria. 2010. Thrombin-Binding Aptamer Quadruplex Formation : AFM and Voltammetric Characterization. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-502548
Modern Language Association (MLA)
Diculescu, Victor Constantin…[et al.]. Thrombin-Binding Aptamer Quadruplex Formation : AFM and Voltammetric Characterization. Journal of Nucleic Acids No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-502548
American Medical Association (AMA)
Diculescu, Victor Constantin& Chiorcea-Paquim, Ana-Maria& Eritja, Ramon& Oliveira-Brett, Ana Maria. Thrombin-Binding Aptamer Quadruplex Formation : AFM and Voltammetric Characterization. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-502548
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502548