Leptin Regulates Proliferation and Apoptosis in Human Prostate
Joint Authors
Cavalcante, Fernanda Silveira
Leze, Eduardo
Colli, Sicilia
da Fonte Ramos, Cristiane
Alves-Pereira, Jorge Luiz
José Sampaio, Francisco
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
This paper aimed to evaluate the leptin role on the cellular proliferation and the expression of fibroblast growth factor 2, aromatase enzyme, and apoptotic genes in the human prostate tissue.
Methods.
Fifteen samples of hyperplasic prostate tissue were divided in four symmetric parts maintained in RPMI medium supplemented with 10% fetal bovine serum, 1 ng/mL of gentamicin, and added with 50 ng/mL leptin (L) or not (C).
After 3 hours of incubation, gene expression was evaluated by real time RT-PCR.
Cellular proliferation was evaluated by immunohistochemistry for PCNA.
Results.
The leptin treatment led to an increase cellular proliferation (C=21.8±0.5; L=64.8±0.9; P<0.0001) and in the expression of Bax (C=0.4±0.1; L=0.9±0.2; P<0.05) while Bcl-2 (C=19.9±5.6; L=5.6±1.8; P<0.05), Bcl-x (C=0.2±0.06; L=0.07±0.02; P<0.05), and aromatase expressions (C=1.9±0.6; L=0.4±0.1; P<0.04) were significantly reduced.
Conclusion.
Leptin has an important role in maintaining the physiological growth of the prostate since it stimulates both cellular proliferation and apoptosis, with the decrement in the aromatase gene expression.
American Psychological Association (APA)
Leze, Eduardo& Alves-Pereira, Jorge Luiz& Colli, Sicilia& Cavalcante, Fernanda Silveira& José Sampaio, Francisco& da Fonte Ramos, Cristiane. 2012. Leptin Regulates Proliferation and Apoptosis in Human Prostate. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-502575
Modern Language Association (MLA)
Leze, Eduardo…[et al.]. Leptin Regulates Proliferation and Apoptosis in Human Prostate. The Scientific World Journal No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-502575
American Medical Association (AMA)
Leze, Eduardo& Alves-Pereira, Jorge Luiz& Colli, Sicilia& Cavalcante, Fernanda Silveira& José Sampaio, Francisco& da Fonte Ramos, Cristiane. Leptin Regulates Proliferation and Apoptosis in Human Prostate. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-502575
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502575