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Strategies Aimed at Nox4 Oxidase Inhibition Employing Peptides from Nox4 B-Loop and C-Terminus and p22phox N-Terminus : An Elusive Target
Joint Authors
Pagano, Patrick J.
Csányi, Gábor
Source
International Journal of Hypertension
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Although NADPH oxidase 4 (Nox4) is the most abundant Nox isoform in systemic vascular endothelial and smooth muscle cells, its function in the vascular tissue is not entirely known.
The literature describes a pathophysiological role for Nox4 in cardiovascular disease; however, some studies have reported that it has a protective role.
To date, specific Nox4 inhibitors are not available; hence, the development of a pharmacologic tool to assess Nox4’s pathophysiological role garners intense interest.
In this study, we selected peptides corresponding to regions in the Nox4 oxidase complex critical to holoenzyme activity and postulated their utility as specific competitive inhibitors.
Previous studies in our laboratory yielded selective inhibition of Nox2 using this strategy.
We postulated that peptides mimicking the Nox4 B-loop and C-terminus and regions on p22phox inhibit Nox4 activity.
To test our hypothesis, the inhibitory activity of Nox4 B-loop and C-terminal peptides as well as N-terminal p22phox peptides was assessed in a reconstituted Nox4 system.
Our findings demonstrate that Nox4 inhibition is not achieved by preincubation with this comprehensive array of peptides derived from previously identified active regions.
These findings suggest that Nox4 exists in a tightly assembled and active conformation which, unlike other Noxes, cannot be disrupted by conventional means.
American Psychological Association (APA)
Csányi, Gábor& Pagano, Patrick J.. 2013. Strategies Aimed at Nox4 Oxidase Inhibition Employing Peptides from Nox4 B-Loop and C-Terminus and p22phox N-Terminus : An Elusive Target. International Journal of Hypertension،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-502647
Modern Language Association (MLA)
Csányi, Gábor& Pagano, Patrick J.. Strategies Aimed at Nox4 Oxidase Inhibition Employing Peptides from Nox4 B-Loop and C-Terminus and p22phox N-Terminus : An Elusive Target. International Journal of Hypertension No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-502647
American Medical Association (AMA)
Csányi, Gábor& Pagano, Patrick J.. Strategies Aimed at Nox4 Oxidase Inhibition Employing Peptides from Nox4 B-Loop and C-Terminus and p22phox N-Terminus : An Elusive Target. International Journal of Hypertension. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-502647
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502647