Characterization of Mononucleated Human Peripheral Blood Cells
Joint Authors
Ab Kadir, Ruzanna
Megat Abdul Wahab, Rohaya
Senafi, Shahidan
Kermani, Shabnam
Zainal Ariffin, Shahrul Hisham
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Unspecialized cells that can renew themselves and give rise to multiple differentiated cell types are termed stem cells.
The objective of this study was to characterize and investigate, through molecular and biochemical analyses, the stemness of cells derived from isolated mononucleated cells that originated from peripheral blood.
The isolated mononucleated cells were separated according to their physical characteristics (adherent and suspension), after 4 to 7 days into a 14-day culturing period in complete medium.
Our results revealed that adherent and suspension cells were positive for mesenchymal stem cell (MSC) and hematopoietic stem cell (HSC) markers, respectively.
Differentiation of adherent cells into osteoblasts was associated with expression of the OPN gene and increasing ALP enzyme activity, while differentiation of suspension cells into osteoclasts was associated with expression of the TRAP gene and increasing TRAP enzyme activity.
In conclusion, molecular and biochemical analyses showed that mononucleated cells consist of MSC (adherent) and HSC (suspension), and both cell types are able to differentiate into specialized cells from their respective lineage: osteoblast (MSC) and osteoclast (HSC).
American Psychological Association (APA)
Ab Kadir, Ruzanna& Zainal Ariffin, Shahrul Hisham& Megat Abdul Wahab, Rohaya& Kermani, Shabnam& Senafi, Shahidan. 2012. Characterization of Mononucleated Human Peripheral Blood Cells. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-502750
Modern Language Association (MLA)
Ab Kadir, Ruzanna…[et al.]. Characterization of Mononucleated Human Peripheral Blood Cells. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-502750
American Medical Association (AMA)
Ab Kadir, Ruzanna& Zainal Ariffin, Shahrul Hisham& Megat Abdul Wahab, Rohaya& Kermani, Shabnam& Senafi, Shahidan. Characterization of Mononucleated Human Peripheral Blood Cells. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-502750
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502750