Morphofunctional and Biochemical Approaches for Studying Mitochondrial Changes during Myoblasts Differentiation
Joint Authors
Battistelli, Michela
Vallorani, Luciana
Polidori, Emanuela
Gioacchini, Anna Maria
Falcieri, Elisabetta
Casadei, Lucia
Zeppa, Sabrina
Piccoli, Giovanni
Stocchi, Vilberto
Barbieri, Elena
Stocchi, Laura
Ceccaroli, Paola
Guescini, Michele
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-10
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Pharmacy, Health & Medical Sciences
Abstract EN
This study describes mitochondrial behaviour during the C2C12 myoblast differentiation program and proposes a proteomic approach to mitochondria integrated with classical morphofunctional and biochemical analyses.
Mitochondrial ultrastructure variations were determined by transmission electron microscopy; mitochondrial mass and membrane potential were analysed by Mitotracker Green and JC-1 stains and by epifluorescence microscope.
Expression of PGC1α, NRF1α, and Tfam genes controlling mitochondrial biogenesis was studied by real-time PCR.
The mitochondrial functionality was tested by cytochrome c oxidase activity and COXII expression.
Mitochondrial proteomic profile was also performed.
These assays showed that mitochondrial biogenesis and activity significantly increase in differentiating myotubes.
The proteomic profile identifies 32 differentially expressed proteins, mostly involved in oxidative metabolism, typical of myotubes formation.
Other notable proteins, such as superoxide dismutase (MnSOD), a cell protection molecule, and voltage-dependent anion-selective channel protein (VDAC1) involved in the mitochondria-mediated apoptosis, were found to be regulated by the myogenic process.
The integration of these approaches represents a helpful tool for studying mitochondrial dynamics, biogenesis, and functionality in comparative surveys on mitochondrial pathogenic or senescent satellite cells.
American Psychological Association (APA)
Barbieri, Elena& Battistelli, Michela& Casadei, Lucia& Vallorani, Luciana& Piccoli, Giovanni& Guescini, Michele…[et al.]. 2011. Morphofunctional and Biochemical Approaches for Studying Mitochondrial Changes during Myoblasts Differentiation. Journal of Aging Research،Vol. 2011, no. 2011, pp.1-16.
https://search.emarefa.net/detail/BIM-502794
Modern Language Association (MLA)
Barbieri, Elena…[et al.]. Morphofunctional and Biochemical Approaches for Studying Mitochondrial Changes during Myoblasts Differentiation. Journal of Aging Research No. 2011 (2011), pp.1-16.
https://search.emarefa.net/detail/BIM-502794
American Medical Association (AMA)
Barbieri, Elena& Battistelli, Michela& Casadei, Lucia& Vallorani, Luciana& Piccoli, Giovanni& Guescini, Michele…[et al.]. Morphofunctional and Biochemical Approaches for Studying Mitochondrial Changes during Myoblasts Differentiation. Journal of Aging Research. 2011. Vol. 2011, no. 2011, pp.1-16.
https://search.emarefa.net/detail/BIM-502794
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502794