Size of AT(n) Insertions in Promoter Region Modulates Gmhsp17.6-L mRNA Transcript Levels
Joint Authors
Silva, João Flávio Veloso
Marin, Silvana Regina Rockenbach
Nepomuceno, Alexandre Lima
Lopes, Valéria Stefania
Marcelino, Francismar Côrrea
Machado, Maria de Fátima Pires da Silva
Abdelnoor, Ricardo Vilela
Binneck, Eliseu
Fuganti, Renata
Arias, Carlos Alberto Arrabal
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
During earlier experiments, an SSR molecular marker (176 Soy HSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean.
After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene.
It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9).
Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes.
A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes.
A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions.
These results suggest that the number of AT(n) insertions inside this promoter region could modulate up or down gene levels.
Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions.
American Psychological Association (APA)
Fuganti, Renata& Machado, Maria de Fátima Pires da Silva& Lopes, Valéria Stefania& Silva, João Flávio Veloso& Arias, Carlos Alberto Arrabal& Marin, Silvana Regina Rockenbach…[et al.]. 2010. Size of AT(n) Insertions in Promoter Region Modulates Gmhsp17.6-L mRNA Transcript Levels. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-502986
Modern Language Association (MLA)
Fuganti, Renata…[et al.]. Size of AT(n) Insertions in Promoter Region Modulates Gmhsp17.6-L mRNA Transcript Levels. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-502986
American Medical Association (AMA)
Fuganti, Renata& Machado, Maria de Fátima Pires da Silva& Lopes, Valéria Stefania& Silva, João Flávio Veloso& Arias, Carlos Alberto Arrabal& Marin, Silvana Regina Rockenbach…[et al.]. Size of AT(n) Insertions in Promoter Region Modulates Gmhsp17.6-L mRNA Transcript Levels. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-502986
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502986