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Role of Linkers between Zinc Fingers in Spacing Recognition by Plant TFIIIA-Type Zinc-Finger Proteins
Joint Authors
Fukushima, Setsuko
Yoshida, Michiteru
Takatsuji, Hiroshi
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The EPF family of plant TFIIIA-type zinc-finger (ZF) proteins (ZPTs) is characterized by long linkers separating ZF motifs.
We previously reported that two-fingered ZPTs bind to two tandem core sites that are separated by several base pairs, each ZF making contact with one core site.
Here we report further characterization of DNA-binding activities of ZPTs using four family members, ZPT2-14, ZPT2-7, ZPT2-8, and ZPT2-2, having inter-ZF linkers of different lengths and sequences, to investigate the correlation of the length and/or sequence of the linker with preference for the spacing between core sites in target DNAs.
Selected and amplified binding site (SAAB)-imprinting assays and gel mobility shift assays prompted three conclusions.
(1) The four ZPTs have common specificity for core binding sites—two AGT(G)/(C)ACTs separated by several nucleotides.
(2) The four ZPTs prefer a spacing of 10 bases between the core sites, but each ZPT has its own preference for suboptimal spacing.
(3) At a particular spacing, two zinc fingers may bind to the core sites on both strands.
The results provide new information about how the diversity in linker length/sequence affects DNA-sequence recognition in this protein family.
American Psychological Association (APA)
Fukushima, Setsuko& Yoshida, Michiteru& Takatsuji, Hiroshi. 2011. Role of Linkers between Zinc Fingers in Spacing Recognition by Plant TFIIIA-Type Zinc-Finger Proteins. Journal of Amino Acids،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-503011
Modern Language Association (MLA)
Fukushima, Setsuko…[et al.]. Role of Linkers between Zinc Fingers in Spacing Recognition by Plant TFIIIA-Type Zinc-Finger Proteins. Journal of Amino Acids No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-503011
American Medical Association (AMA)
Fukushima, Setsuko& Yoshida, Michiteru& Takatsuji, Hiroshi. Role of Linkers between Zinc Fingers in Spacing Recognition by Plant TFIIIA-Type Zinc-Finger Proteins. Journal of Amino Acids. 2011. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-503011
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503011