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Heterologous Expression of a Membrane-Spanning Auxin Importer : Implications for Functional Analyses of Auxin Transporters
Joint Authors
Dorrian, James Matthew
Carrier, David John
Lawler, Karen
Kerr, Ian Derek
Abu Bakar, Norliza Tendot
Bennett, Malcolm John
Haider, Ameena
Source
International Journal of Plant Genomics
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-06-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Biochemical studies of plant auxin transporters in vivo are made difficult by the presence of multiple auxin transporters and auxin-interacting proteins.
Furthermore, the expression level of most such transporters in plants is likely to be too low for purification and downstream functional analysis.
Heterologous expression systems should address both of these issues.
We have examined a number of such systems for their efficiency in expressing AUX1 from Arabidopsis thaliana.
We find that a eukaryotic system based upon infection of insect cells with recombinant baculovirus provides a high level, easily scalable expression system capable of delivering a functional assay for AUX1.
Furthermore, a transient transfection system in mammalian cells enables localization of AUX1 and AUX1-mediated transport of auxin to be investigated.
In contrast, we were unable to utilise P.
pastoris or L.
lactis expression systems to reliably express AUX1.
American Psychological Association (APA)
Carrier, David John& Abu Bakar, Norliza Tendot& Lawler, Karen& Dorrian, James Matthew& Haider, Ameena& Bennett, Malcolm John…[et al.]. 2009. Heterologous Expression of a Membrane-Spanning Auxin Importer : Implications for Functional Analyses of Auxin Transporters. International Journal of Plant Genomics،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-503021
Modern Language Association (MLA)
Carrier, David John…[et al.]. Heterologous Expression of a Membrane-Spanning Auxin Importer : Implications for Functional Analyses of Auxin Transporters. International Journal of Plant Genomics No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-503021
American Medical Association (AMA)
Carrier, David John& Abu Bakar, Norliza Tendot& Lawler, Karen& Dorrian, James Matthew& Haider, Ameena& Bennett, Malcolm John…[et al.]. Heterologous Expression of a Membrane-Spanning Auxin Importer : Implications for Functional Analyses of Auxin Transporters. International Journal of Plant Genomics. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-503021
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503021