![](/images/graphics-bg.png)
Development and Retranslational Validation of an In Vitro Model to Characterize Acute Infections in Large Human Joints
Joint Authors
Südkamp, Norbert P.
Mehlhorn, Alexander T.
Pilz, Ingo H.
Dovi-Akue, David
Langenmair, Elia Raoul
Schmal, Hagen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Bacterial infections can destroy cartilage integrity, resulting in osteoarthritis.
Goal was to develop an in vitro model with in vivo validation of acute joint inflammation.
Inflammation in cocultivated human synovial fibroblasts (SFB), chondrocytes (CHDR), and mononuclear cells (MNC) was successively relieved for 10 days.
Articular effusions from patients with (n=7) and without (n=5) postoperative joint infection in healthy patients (ASA 1-2) were used as model validation.
Inflammation in vitro resulted in an enormous increase in IL-1 and a successive reduction in SFB numbers.
CHDR however, maintained metabolic activity and proteoglycan synthesis.
While concentrations of bFGF in vivo and in vitro rose consistently, the mRNA increase was only moderate.
Concurring with our in vivo data, cartilage-specific IGF-1 steadily increased, while IGF-1 mRNA in the CHDR and SFB did not correlate with protein levels.
Similarly, aggrecan (ACAN) protein concentrations increased in vivo and failed to correlate in vitro with gene expression in either the CHDR or the SFB, indicating extracellular matrix breakdown.
Anabolic cartilage-specific BMP-7 with highly significant intra-articular levels was significantly elevated in vitro on day 10 following maximum inflammation.
Our in vitro model enables us to validate early inflammation of in vivo cell- and cytokine-specific regulatory patterns.
This trial is registered with MISSinG, DRKS 00003536.
American Psychological Association (APA)
Pilz, Ingo H.& Mehlhorn, Alexander T.& Dovi-Akue, David& Langenmair, Elia Raoul& Südkamp, Norbert P.& Schmal, Hagen. 2014. Development and Retranslational Validation of an In Vitro Model to Characterize Acute Infections in Large Human Joints. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-503054
Modern Language Association (MLA)
Pilz, Ingo H.…[et al.]. Development and Retranslational Validation of an In Vitro Model to Characterize Acute Infections in Large Human Joints. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-503054
American Medical Association (AMA)
Pilz, Ingo H.& Mehlhorn, Alexander T.& Dovi-Akue, David& Langenmair, Elia Raoul& Südkamp, Norbert P.& Schmal, Hagen. Development and Retranslational Validation of an In Vitro Model to Characterize Acute Infections in Large Human Joints. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-503054
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503054