Aberrant Methylation Inactivates Transforming Growth Factor β Receptor I in Head and Neck Squamous Cell Carcinoma
Joint Authors
Báez, Adriana
Mathews, Linda A.
Clavell, Jonathan
Torrellas-Ruiz, Mariclara
Muñoz-Antonia, Teresita
Muro-Cacho, Carlos A.
Source
International Journal of Otolaryngology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-06-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Alterations in TGF-β signaling are common in head and neck cancer (HNSCC).
Mutations in TGF-β type II receptor (TβR-II) occur frequently in HNSCC while TGF-β type I receptor (TβR-I) mutations are rare, suggesting that other molecular alterations in the TGF-β pathway are likely.
To identify abnormalities in TβR-I expression we analyzed 50 HNSCCs and correlated the results with clinical-pathologic features.
Methods.
Hypermethylation of TβR-I was evaluated via methylation-specific PCR (MSP) and restriction enzyme-mediated PCR (MSRE).
Mutations in exons 1 and 7, mRNA and protein expression were analyzed by direct sequencing, semiquantitative RT—PCR and immunohistochemistry, respectively.
Results.
TβR-I expression was lost in 83% HNSCCs and was linked to DNA hypermethylation of the CpG-rich promoter region in 62% of the tumors.
The variants 9A/6A and Int7G24A were found in two patients.
Conclusions.
This study shows that suppression of TβR-I expression in HNSCC is associated with DNA hypermethylation.
American Psychological Association (APA)
Muñoz-Antonia, Teresita& Torrellas-Ruiz, Mariclara& Clavell, Jonathan& Mathews, Linda A.& Muro-Cacho, Carlos A.& Báez, Adriana. 2009. Aberrant Methylation Inactivates Transforming Growth Factor β Receptor I in Head and Neck Squamous Cell Carcinoma. International Journal of Otolaryngology،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-503066
Modern Language Association (MLA)
Muñoz-Antonia, Teresita…[et al.]. Aberrant Methylation Inactivates Transforming Growth Factor β Receptor I in Head and Neck Squamous Cell Carcinoma. International Journal of Otolaryngology No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-503066
American Medical Association (AMA)
Muñoz-Antonia, Teresita& Torrellas-Ruiz, Mariclara& Clavell, Jonathan& Mathews, Linda A.& Muro-Cacho, Carlos A.& Báez, Adriana. Aberrant Methylation Inactivates Transforming Growth Factor β Receptor I in Head and Neck Squamous Cell Carcinoma. International Journal of Otolaryngology. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-503066
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503066