Subcellular Microanatomy by 3D Deconvolution Brightfield Microscopy : Method and Analysis Using Human Chromatin in the Interphase Nucleus

Abstract EN

Anatomy has advanced using 3-dimensional (3D) studies at macroscopic (e.g., dissection, injection moulding of vessels, radiology) and microscopic (e.g., serial section reconstruction with light and electron microscopy) levels.

This paper presents the first results in human cells of a new method of subcellular 3D brightfield microscopy.

Unlike traditional 3D deconvolution and confocal techniques, this method is suitable for general application to brightfield microscopy.

Unlike brightfield serial sectioning it has subcellular resolution.

Results are presented of the 3D structure of chromatin in the interphase nucleus of two human cell types, hepatocyte and plasma cell.

I show how the freedom to examine these structures in 3D allows greater morphological discrimination between and within cell types and the 3D structural basis for the classical “clock-face” motif of the plasma cell nucleus is revealed.

Potential for further applications discussed.