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High-Throughput Peptide Epitope Mapping Using Carbon Nanotube Field-Effect Transistors
Joint Authors
Knight, Martha
Kwon, Hena H.
Stefansson, Lára A.
Ahn, Saeyoung Nate
Stefansson, Steingrimur
Source
International Journal of Peptides
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Label-free and real-time detection technologies can dramatically reduce the time and cost of pharmaceutical testing and development.
However, to reach their full promise, these technologies need to be adaptable to high-throughput automation.
To demonstrate the potential of single-walled carbon nanotube field-effect transistors (SWCNT-FETs) for high-throughput peptide-based assays, we have designed circuits arranged in an 8 × 12 (96-well) format that are accessible to standard multichannel pipettors.
We performed epitope mapping of two HIV-1 gp160 antibodies using an overlapping gp160 15-mer peptide library coated onto nonfunctionalized SWCNTs.
The 15-mer peptides did not require a linker to adhere to the non-functionalized SWCNTs, and binding data was obtained in real time for all 96 circuits.
Despite some sequence differences in the HIV strains used to generate these antibodies and the overlapping peptide library, respectively, our results using these antibodies are in good agreement with known data, indicating that peptides immobilized onto SWCNT are accessible and that linear epitope mapping can be performed in minutes using SWCNT-FET.
American Psychological Association (APA)
Stefansson, Steingrimur& Knight, Martha& Kwon, Hena H.& Stefansson, Lára A.& Ahn, Saeyoung Nate. 2013. High-Throughput Peptide Epitope Mapping Using Carbon Nanotube Field-Effect Transistors. International Journal of Peptides،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-503108
Modern Language Association (MLA)
Stefansson, Steingrimur…[et al.]. High-Throughput Peptide Epitope Mapping Using Carbon Nanotube Field-Effect Transistors. International Journal of Peptides No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-503108
American Medical Association (AMA)
Stefansson, Steingrimur& Knight, Martha& Kwon, Hena H.& Stefansson, Lára A.& Ahn, Saeyoung Nate. High-Throughput Peptide Epitope Mapping Using Carbon Nanotube Field-Effect Transistors. International Journal of Peptides. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-503108
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503108