Selective Incision of the α-N5-Methyl-Formamidopyrimidine Anomer by Escherichia coli Endonuclease IV
Joint Authors
Banerjee, Surajit
Rizzo, Carmelo J.
Stone, Michael P.
Christov, Plamen P.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Formamidopyrimidines (Fapy) lesions result from ring opening of the imidazole portion of purines.
Fapy lesions can isomerize from the natural β-anomeric stereochemistry to the α-configuration.
We have unambiguously demonstrated that the α-methyl-Fapy-dG (MeFapy-dG) lesion is a substrate for Escherichia coli Endonuclease IV (Endo IV).
Treatment of a MeFapy-dG-containing 24 mer duplex with Endo IV resulted in 36–40% incision.
The catalytic efficiency of the incision was comparable to that of α-dG in the same duplex sequence.
The α- and β-MeFapy-dG anomers equilibrate to ~21 : 79 ratio over ~3 days.
Related studies with a duplex containing the α-Fapy-dG lesion derived from aflatoxin B1 epoxide (α-AFB-Fapy-dG) showed only low levels of incision.
It is hypothesized that the steric bulk of the aflatoxin moiety interferes with the binding of the substrate to Endo IV and the incision chemistry.
American Psychological Association (APA)
Christov, Plamen P.& Banerjee, Surajit& Stone, Michael P.& Rizzo, Carmelo J.. 2010. Selective Incision of the α-N5-Methyl-Formamidopyrimidine Anomer by Escherichia coli Endonuclease IV. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-503190
Modern Language Association (MLA)
Christov, Plamen P.…[et al.]. Selective Incision of the α-N5-Methyl-Formamidopyrimidine Anomer by Escherichia coli Endonuclease IV. Journal of Nucleic Acids No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-503190
American Medical Association (AMA)
Christov, Plamen P.& Banerjee, Surajit& Stone, Michael P.& Rizzo, Carmelo J.. Selective Incision of the α-N5-Methyl-Formamidopyrimidine Anomer by Escherichia coli Endonuclease IV. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-503190
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503190