Identification of Multiple Soluble Fe(III) Reductases in Gram-Positive Thermophilic Bacterium Thermoanaerobacter indiensis BSB-33
Author
Source
International Journal of Genomics
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Thermoanaerobacter indiensis BSB-33 has been earlier shown to reduce Fe(III) and Cr(VI) anaerobically at 60°C optimally.
Further, the Gram-positive thermophilic bacterium contains Cr(VI) reduction activity in both the membrane and cytoplasm.
The soluble fraction prepared from T.
indiensis cells grown at 60°C was found to contain the majority of Fe(III) reduction activity of the microorganism and produced four distinct bands in nondenaturing Fe(III) reductase activity gel.
Proteins from each of these bands were partially purified by chromatography and identified by mass spectrometry (MS) with the help of T.
indiensis proteome sequences.
Two paralogous dihydrolipoamide dehydrogenases (LPDs), thioredoxin reductase (Trx), NADP(H)-nitrite reductase (Ntr), and thioredoxin disulfide reductase (Tdr) were determined to be responsible for Fe(III) reductase activity.
Amino acid sequence and three-dimensional (3D) structural similarity analyses of the T.
indiensis Fe(III) reductases were carried out with Cr(VI) reducing proteins from other bacteria.
The two LPDs and Tdr showed very significant sequence and structural identity, respectively, with Cr(VI) reducing dihydrolipoamide dehydrogenase from Thermus scotoductus and thioredoxin disulfide reductase from Desulfovibrio desulfuricans.
It appears that in addition to their iron reducing activity T.
indiensis LPDs and Tdr are possibly involved in Cr(VI) reduction as well.
American Psychological Association (APA)
Pal, Subrata. 2014. Identification of Multiple Soluble Fe(III) Reductases in Gram-Positive Thermophilic Bacterium Thermoanaerobacter indiensis BSB-33. International Journal of Genomics،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-503233
Modern Language Association (MLA)
Pal, Subrata. Identification of Multiple Soluble Fe(III) Reductases in Gram-Positive Thermophilic Bacterium Thermoanaerobacter indiensis BSB-33. International Journal of Genomics No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-503233
American Medical Association (AMA)
Pal, Subrata. Identification of Multiple Soluble Fe(III) Reductases in Gram-Positive Thermophilic Bacterium Thermoanaerobacter indiensis BSB-33. International Journal of Genomics. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-503233
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503233