Preventive Effects of Epigallocatechin-3-O-Gallate against Replicative Senescence Associated with p53 Acetylation in Human Dermal Fibroblasts
Joint Authors
Hyon, Suong-Hyu
Han, Dong-Wook
Lee, Mi Hee
Lee, Jun Jae
Kim, Bongju
Park, Jong-Chul
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-18
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Considering the various pharmacological activities of epigallocatechin-3-O-gallate (EGCG) including anticancer, and anti-inflammatory, antidiabetic, and so forth, relatively less attention has been paid to the antiaging effect of EGCG on primary cells.
In this study, the preventive effects of EGCG against serial passage-induced senescence were investigated in primary cells including rat vascular smooth muscle cells (RVSMCs), human dermal fibroblasts (HDFs), and human articular chondrocytes (HACs).
The involvement of Sirt1 and acetylated p53 was examined as an underlying mechanism for the senescence preventive activity of EGCG in HDFs.
All cells were employed with the initial passage number (PN) between 3 and 7.
For inducing senescence, the cells were serially passaged at the predetermined times and intervals in the absence or presence of EGCG (50 or 100 μM).
Serial passage-induced senescence in RVSMCs and HACs was able to be significantly prevented at 50 μM EGCG, while in HDFs, 100 μM EGCG could significantly prevent senescence and recover their cell cycle progression close to the normal level.
Furthermore, EGCG was found to prevent serial passage- and H2O2-induced senescence in HDFs by suppressing p53 acetylation, but the Sirt1 activity was unaffected.
In addition, proliferating HDFs showed similar cellular uptake of FITC-conjugated EGCG into the cytoplasm with their senescent counterparts but different nuclear translocation of it from them, which would partly account for the differential responses to EGCG in proliferating versus senescent cells.
Taking these results into consideration, it is suggested that EGCG may be exploited to craft strategies for the development of an antiaging or age-delaying agent.
American Psychological Association (APA)
Han, Dong-Wook& Lee, Mi Hee& Kim, Bongju& Lee, Jun Jae& Hyon, Suong-Hyu& Park, Jong-Chul. 2012. Preventive Effects of Epigallocatechin-3-O-Gallate against Replicative Senescence Associated with p53 Acetylation in Human Dermal Fibroblasts. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-503242
Modern Language Association (MLA)
Han, Dong-Wook…[et al.]. Preventive Effects of Epigallocatechin-3-O-Gallate against Replicative Senescence Associated with p53 Acetylation in Human Dermal Fibroblasts. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-503242
American Medical Association (AMA)
Han, Dong-Wook& Lee, Mi Hee& Kim, Bongju& Lee, Jun Jae& Hyon, Suong-Hyu& Park, Jong-Chul. Preventive Effects of Epigallocatechin-3-O-Gallate against Replicative Senescence Associated with p53 Acetylation in Human Dermal Fibroblasts. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-503242
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503242