Cathepsin G, a Neutrophil Protease, Induces Compact Cell-Cell Adhesion in MCF-7 Human Breast Cancer Cells
Joint Authors
Kudo, Tomoya
Yui, Satoru
Yamazaki, Masatoshi
Takino, Takahisa
Kigoshi, Hideaki
Hagiwara, Takashi
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Cathepsin G is a serine protease secreted by activated neutrophils that play a role in the inflammatory response.
Because neutrophils are known to be invading leukocytes in various tumors, their products may influence the characteristics of tumor cells such as the growth state, motility, and the adhesiveness between cells or the extracellular matrix.
Here, we demonstrate that cathepsin G induces cell-cell adhesion of MCF-7 human breast cancer cells resulting from the contact inhibition of cell movement on fibronectin but not on type IV collagen.
Cathepsin G subsequently induced cell condensation, a very compact cell colony, resulting due to the increased strength of E-cadherin-mediated cell-cell adhesion.
Cathepsin G action is protease activity-dependent and was inhibited by the presence of serine protease inhibitors.
Cathepsin G promotes E-cadherin/catenin complex formation and Rap1 activation in MCF-7 cells, which reportedly regulates E-cadherin-based cell-cell junctions.
Cathepsin G also promotes E-cadherin/protein kinase D1 (PKD1) complex formation, and Go6976, the selective PKD1 inhibitor, suppressed the cathepsin G-induced cell condensation.
Our findings provide the first evidence that cathepsin G regulates E-cadherin function, suggesting that cathepsin G has a novel modulatory role against tumor cell-cell adhesion.
American Psychological Association (APA)
Kudo, Tomoya& Kigoshi, Hideaki& Hagiwara, Takashi& Takino, Takahisa& Yamazaki, Masatoshi& Yui, Satoru. 2009. Cathepsin G, a Neutrophil Protease, Induces Compact Cell-Cell Adhesion in MCF-7 Human Breast Cancer Cells. Mediators of Inflammation،Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-503268
Modern Language Association (MLA)
Kudo, Tomoya…[et al.]. Cathepsin G, a Neutrophil Protease, Induces Compact Cell-Cell Adhesion in MCF-7 Human Breast Cancer Cells. Mediators of Inflammation No. 2009 (2009), pp.1-11.
https://search.emarefa.net/detail/BIM-503268
American Medical Association (AMA)
Kudo, Tomoya& Kigoshi, Hideaki& Hagiwara, Takashi& Takino, Takahisa& Yamazaki, Masatoshi& Yui, Satoru. Cathepsin G, a Neutrophil Protease, Induces Compact Cell-Cell Adhesion in MCF-7 Human Breast Cancer Cells. Mediators of Inflammation. 2009. Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-503268
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503268